The immunodominant epitope of centromere-associated protein A displays homology with the transcription factor forkhead box E3 (FOXE3)

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Federico PerosaFranco Dammacco

Abstract

Some patients with systemic sclerosis express autoantibodies to centromere-associated protein A (CENP-A), but the exact CENP-A epitope is unknown and it is possible that another protein primes these antibodies. This study aimed to define the amino acids recognized by these antibodies and discover other proteins that may be targeted by or may prime them. Peptide Ap(17-30), the immunodominant epitope of CENP-A, was used to purify anti-CENP-A Ig from sera of 8 patients. Anti-Ap(17-30) Ig reacted dose-dependently with Ap(17-30). Panning a phage display peptide library with anti-Ap(17-30) IgG identified two overlapping motifs (m), pt1m and pt8m, permitting patients classification into subgroups based on their having antibodies for only pt1m, pt8m, or both. The pt1m matched residues 53-62 of forkhead box E3; this peptide (FOXE3p(53-62)) behaved similarly in binding and inhibition assays with anti-Ap(17-30) IgG and elicited anti-Ap(17-30) antibodies in mice. This study sets the ground for future investigations on a possible relationship between antibody specificity and clinical manifestations of systemic sclerosis, as well as on a possible role of FOXE3 in priming these antibodies.

References

Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W C EarnshawD W Cleveland
Feb 1, 1986·The Journal of Clinical Investigation·W EarnshawN Rothfield
Aug 1, 1996·Arthritis and Rheumatism·J B RattnerM J Fritzler
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·K YodaT Okazaki
Nov 30, 2000·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M MahlerM Blüthner
Jul 19, 2002·Arthritis and Rheumatism·Lionel SchachnaLivia Casciola-Rosen
Jun 13, 2003·Trends in Genetics : TIG·Ordan J LehmannShomi S Bhattacharya
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico PerosaFranco Dammacco
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Massimo MaddaloniSeth H Pincus
May 5, 2005·Molecular and Cellular Biology·Vinciane RégnierWilliam Brown
Jan 11, 2007·Journal of Autoimmunity·Yasmin AkbaraliJudith A James
Mar 3, 2007·The Journal of Clinical Investigation·John Varga, David Abraham
Mar 9, 2007·Development·Olga Medina-Martinez, Milan Jamrich
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico PerosaFranco Dammacco
Jan 29, 2008·Seminars in Arthritis and Rheumatism·Y Stephanie GuM Eric Gershwin
Feb 21, 2008·Nature Immunology·Qizhi Tang, Jeffrey A Bluestone
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico PerosaFranco Dammacco
May 8, 2009·The New England Journal of Medicine·Armando GabrielliThomas Krieg
Aug 1, 2009·Arthritis and Rheumatism·Tomeka Suber, Antony Rosen

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