The immunogenic properties of poly(dI).poly(dC) and poly(rI). poly(dC)--analysis by monoclonal antibodies

Molecular Immunology
R P BraunJ S Lee

Abstract

Eight monoclonal antibodies were prepared from a C57/black mouse which had been immunized with poly(rI).poly(dC). Two of the antibodies were specific for the RNA-DNA duplex but bound about 100-fold better to poly(rI).poly(dC) than to poly(rA).poly(dT). The other six antibodies were single-strand specific and bound to poly(rI) and in most cases to poly(dI) as well, but not to other single-stranded nucleic acids. Similarly, 10 monoclonal antibodies were produced from mice immunized with poly(dI).poly(dC). Nine of these were specific for poly(dI) while the other had a strong preference for poly(dC). Thus, in contrast to poly(rI).poly(dC), the all DNA duplex poly(dI). poly(dC) only elicits duplex specific antibodies at very low frequency, if at all.

References

Oct 10, 1979·Nucleic Acids Research·A R MorganD H Evans
Oct 7, 1985·FEBS Letters·J S LeeM L Woodsworth
Jun 5, 1985·Journal of Molecular Biology·M McCallO Kennard
Sep 25, 1980·Nucleic Acids Research·J S LeeA R Morgan
Mar 26, 1984·FEBS Letters·J S LeeL J Latimer
Feb 24, 1983·The New England Journal of Medicine·Y ShoenfeldR S Schwartz
Jun 1, 1982·Molecular Immunology·D W Ballard, E W Voss
Oct 15, 1980·Journal of Molecular Biology·A G LeslieR L Ratliff

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Citations

Nov 27, 1998·Nature Biotechnology·M C FletcherJ S Lee
Jul 12, 2014·Expert Review of Molecular Diagnostics·Zonglin HuChristopher A Elkins
Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jing-Dong YeJoseph A Piccirilli
Feb 1, 1997·Molecular Immunology·J TanhaJ S Lee

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