The immunoglobulin G subclass composition of immune complexes in cystic fibrosis. Implications for the pathogenesis of the Pseudomonas lung lesion

The Journal of Clinical Investigation
D B Hornick, R B Fick

Abstract

It has been shown that pulmonary macrophage (PM) phagocytosis of Pseudomonas aeruginosa (PA) is inhibited in the presence of serum from cystic fibrosis (CF) patients colonized by Pseudomonas, and that these sera contain high concentrations of IgG2 antibodies. The goal of these studies was to investigate the role that IgG2-containing immune complexes (IC) play in this inhibition of both PM and neutrophil phagocytosis. We found that serum IgG2 concentrations were elevated significantly in CF patients with chronic PA colonization and that in selected sera from CF patients with chronic PA colonization (CF + IC, n = 10), the mean IC level was significantly elevated (2.90 +/- 0.22 mg/dl [SEM]). IgG2 comprised 74.5% of IgG precipitated in IC from CF + IC sera. An invitro phagocytic assay of [14C]PA uptake using CF + IC whole-sera opsonins confirmed that endocytosis by normal PM and neutrophils was significantly depressed. Removal of IC from CF + IC sera resulted in significantly decreased serum IgG2 concentrations without a significant change in the other subclass concentrations, and enhanced [14C]PA uptake by PM (26.6% uptake increased to 47.3%) and neutrophils (16.9% increased to 52.6%). Return of the soluble IgG2 IC to the original...Continue Reading

References

Sep 9, 1976·The New England Journal of Medicine·P A di Sant'Agnese, P B Davis
Feb 22, 1975·British Medical Journal·H McFarlaneB Worsley
Jun 1, 1980·Pediatric Research·M BerdischewskyE V Barnett
Oct 1, 1981·The Journal of Clinical Investigation·R B FickH Y Reynolds
May 1, 1980·Analytical Biochemistry·S I RennardP G Robey
Jan 1, 1974·The Journal of Pediatrics·H R HillP G Quie

❮ Previous
Next ❯

Citations

Dec 11, 2008·Journal of Gastroenterology and Hepatology·Antonio M Morselli-Labate, Raffaele Pezzilli
Feb 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G KronborgN Høiby
Jun 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M UlanovaN Høiby
Oct 1, 2005·Medicina·Juan Antonio Barcat
Aug 13, 2014·The Journal of Experimental Medicine·Timothy J WellsRobert A Stockley
May 30, 2012·Clinics in Chest Medicine·Grant W Waterer
Jun 26, 2009·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Hiroyuki KobayashiShin Kawai
Nov 24, 2016·The Journal of Chemical Physics·Kento Kasahara, Hirofumi Sato
Mar 3, 2017·The Journal of Chemical Physics·Andrés Montoya-Castillo, David R Reichman
Aug 10, 2005·Treatments in Respiratory Medicine·Hiroyuki Kobayashi
Jul 7, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Chandima DivithotawelaDaniel C Chambers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.