The immunohistochemical detection of J chain in lymphoid cells in tissue sections: the necessity of trypsin digestion

Cellular Immunology
I Hajdu

Abstract

The immunohistochemical detection of J chain in histological sections of tonsils was compared to that of immunoglobulin IgG, IgM, and IgA. Pretreatment of tissue sections with detergents (saponin and Triton X-100) and with proteolytic enzyme (trypsin) at different experimental conditions has shown (i) 0.1% trypsin digestion for 1 hr at room temperature increased dramatically both the number of J-chain-positive cells detected, and the intensity of the color reaction, while the detergents had no noticeable effect; (ii) under the same conditions, trypsin also improved the detection of immunoglobulins, but quantitatively and qualitatively far less effectively than for J chain. The effect of trypsin occurred after 30 min. These results indicate that in tissue sections, the antigenic determinants of J chain are "masked" in the molecule of immunoglobulin in such a manner that the use of trypsin hydrolysis may be important for its successful detection at the light microscopic level. In the case of intact immunoglobulins, this is less important.

Citations

Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·G Kelényi
Jul 1, 1991·Clinics in Dermatology·C C Otley, R P Hall
Jun 1, 1987·Journal of the American Academy of Dermatology·R P Hall

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