The Immunohistochemical Expression of Programmed Death Ligand 1 (PD-L1) Is Affected by Sample Overfixation.

Applied Immunohistochemistry & Molecular Morphology : AIMM
Angels BarberàPedro L Fernández

Abstract

Humanized antibodies targeting programmed death receptor 1 (PD-1) or its ligand (PD-L1) have been approved for the treatment of different cancers. Some of these antibodies show a correlation between the tissue expression of PD-L1 and response. Evaluation of PD-L1 expression presents multiple challenges, but some preanalytical issues such as tissue fixation have been scarcely evaluated. With the hypothesis that immunohistochemical staining of PD-L1 may be impacted by the time of specimen fixation, we evaluated differences in its expression in tonsil samples exposed to predefined fixation times. Random nontumoral tonsillectomy specimens were blindly evaluated in tissue microarray slides after staining with SP142 and SP263 antibodies. With fixation times ranging from 12 to 72 hours, between 2.8% and 6.1% of the samples were considered to be suboptimally stained, with no differences between the 2 antibodies within these fixation times. A significantly higher proportion of samples exposed to a fixation time of 96 hours presented suboptimal immunostaining (15.6%, P<0.0001). In addition, suboptimally stained spots were 20.8% using SP142 and 10.4% using SP263 after 96 hours of fixation (P=0.046). In conclusion, the quality of staining ...Continue Reading

References

Jan 15, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Stéphane ChampiatJean-Charles Soria
Dec 9, 2014·The New England Journal of Medicine·Stephen M AnsellPhilippe Armand
Apr 22, 2015·The New England Journal of Medicine·Michael A PostowF Stephen Hodi
Aug 20, 2016·JAMA Oncology·Patricia GauleDavid L Rimm
Jan 12, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marianne J RatcliffeJill Walker
Jul 19, 2017·Applied Immunohistochemistry & Molecular Morphology : AIMM·Edwin R ParraJaime Rodriguez-Canales
Jul 29, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Chao LiGen Lin
Oct 21, 2017·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Reinhard BüttnerMing-Sound Tsao
Dec 7, 2017·International Journal of Molecular Sciences·Robert J TorphyYuwen Zhu
Jan 19, 2018·Applied Immunohistochemistry & Molecular Morphology : AIMM·Bharathi VennapusaZachary Boyd
Jan 20, 2018·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J AdamS Lantuéjoul
Apr 17, 2018·The New England Journal of Medicine·Leena GandhiUNKNOWN KEYNOTE-189 Investigators
Jun 28, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Christina KoppelBharat Jasani
Aug 18, 2018·Cancer Discovery·Spencer C WeiJames P Allison
Oct 28, 2019·Journal of Pathology and Translational Medicine·Sunhee ChangSe Jin Jang

❮ Previous
Next ❯

Citations

Mar 5, 2021·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Mari Mino-KenudsonSylvie Lantuejoul

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
biopsy

Software Mentioned

R Foundation for Statistical Computing

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.