PMID: 7518368Jul 1, 1994Paper

The immunomodulatory activity of human amniotic fluid can be correlated with transforming growth factor-beta 1 (TGF-beta 1) and beta 2 activity

Clinical and Experimental Immunology
A K Lang, R F Searle

Abstract

The role of alphafetoprotein (AFP) in the immunomodulatory activity of amniotic fluids (AF) from normally progressing human pregnancy (weeks 14-16) was investigated. A panel of 42 AF (25% v/v) reduced significantly phytohaemagglutinin (PHA)-induced peripheral blood mononuclear cell (PBMC) proliferation in serum-free cultures with a mean per cent inhibition of 68.4 +/- 5.5%. In contrast, AFP preparations, with one exception (U.AFP), failed to display inhibitory activity. Pretreatment of AF with anti-TGF-beta 1 and beta 2 antibodies used alone resulted in the mean per cent loss of inhibition of 33.1 +/- 3.9% and 52.3 +/- 7.5%, respectively. A summative loss of AF-mediated inhibition was detected when anti-TGF-beta 1 and beta 2 antibodies were used in combination, but immunomodulation was rarely abolished 100% by this treatment. Anti-TGF-beta 2 antibody treatment, unlike anti-TGF-beta 1 antibody treatment, reversed the inhibitory activity of U.AFP. The amount of TGF-beta 1 and beta 2 contained in human AF was studied by growth inhibition of Mv1 Lu cells. The mean levels of TGF-beta 1 and beta 2 in AF were 11 +/- 0.9 U/ml and 2.3 +/- 0.4 U/ml, respectively, which corresponds with a mean per cent inhibition of 49 +/- 4.7%. U.AFP als...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E P LesterS Yachnin
Feb 15, 1979·American Journal of Obstetrics and Gynecology·N GleicherS B Gusberg
Oct 1, 1992·Clinical Immunology and Immunopathology·H SasakiF Suzuki
Dec 1, 1986·British Journal of Obstetrics and Gynaecology·N S Nicholas, G S Panayi

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Citations

Aug 26, 1998·The Journal of Clinical Endocrinology and Metabolism·P HatthachoteJ I Gillespie
Oct 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stefania ChrissouliDimitris Kletsas
Jun 21, 2001·In Vitro Cellular & Developmental Biology. Animal·C K HoS Y Wang
Sep 1, 1996·American Journal of Reproductive Immunology : AJRI·M D SrivastavaB I Srivastava
Jul 28, 2016·Cell and Tissue Banking·Jan PierceJo-Anna Reems
Jun 24, 2004·American Journal of Reproductive Immunology : AJRI·Kazuhide OgitaYuji Murata
Jul 24, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C A JonesJ O Warner
Jun 21, 2011·American Journal of Reproductive Immunology : AJRI·Steven S WitkinDaniel Skupski
Jul 5, 2003·Placenta·J A KeelanM D Mitchell

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