The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection

BMC Immunology
Khamia RyansQing He

Abstract

We have previously reported that interleukin-10 (IL-10) deficient dendritic cells (DCs) are potent antigen presenting cells that induced elevated protective immunity against Chlamydia. To further investigate the molecular and biochemical mechanism underlying the superior immunostimulatory property of IL-10 deficient DCs we performed proteomic analysis on protein profiles from Chlamydia-pulsed wild-type (WT) and IL-10-/- DCs to identify differentially expressed proteins with immunomodulatory properties. The results showed that alpha enolase (ENO1), a metabolic enzyme involved in the last step of glycolysis was significantly upregulated in Chlamydia-pulsed IL-10-/- DCs compared to WT DCs. We further studied the immunoregulatory role of ENO1 in DC function by generating ENO1 knockdown DCs, using lentiviral siRNA technology. We analyzed the effect of the ENO1 knockdown on DC functions after pulsing with Chlamydia. Pyruvate assay, transmission electron microscopy, flow cytometry, confocal microscopy, cytokine, T-cell activation and adoptive transfer assays were also used to study DC function. The results showed that ENO1 knockdown DCs had impaired maturation and activation, with significant decrease in intracellular pyruvate concent...Continue Reading

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Citations

Feb 17, 2018·Journal of Leukocyte Biology·Federica M Marelli-Berg, Maryam Jangani
May 11, 2019·Frontiers in Immunology·Stefanie K WculekDavid Sancho
Aug 30, 2018·Frontiers in Genetics·Robert B OnzimaRichard P M A Crooijmans
Nov 21, 2018·Frontiers in Immunology·Farhan BasitI Jolanda M de Vries
Jul 1, 2021·Journal of Translational Autoimmunity·Aaron LernerYehuda Shoenfeld

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Methods Mentioned

BETA
transfection
electrophoresis
flow cytometry
Assay
protein profile
protein folding
confocal microscopy
gene knockout
transmission electron microscopy

Software Mentioned

BD Accuri C6
GraphPad Prism
ImageQuant
ImageQuant Reader
ImageQuant TL
MASCOT Daemon
Excel
ImageJ
guavaSoft

Related Concepts