The immunostimulating effect of bacterial genomic DNA on the innate immune responses of bivalve mussel, Hyriopsis cumingii Lea

Fish & Shellfish Immunology
Xu-Tao HongJianzhong Shao

Abstract

The genomic DNA of Escherichia coli, which contains the unmethylated CpG motif, was used to evaluate the immunostimulating effect of bacterial DNA on innate immune responses in the bivalve mussel Hyriopsis cumingii Lea. The results showed that the E. coli DNA had no significant effect on the production of superoxide anion (O(2-)) or acid phosphatase (AP) by haemocytes in vitro. However, the bactericidal activity of the haemocytes was significantly increased when the cells were incubated with 50 or 100mug/ml bacterial DNA for 12 and 24h. Antibacterial activity, lysozyme activity, and prophenoloxidase (proPO) production of haemolymph were also increased, when the bivalve molluscs were injected with 50 or 100mug/ml of bacterial DNA for 12 and 24h. These activities returned to the control level after 48h. This work showed the bacterial DNA with unmethylated CpG motif could activate some parameters of the immune system of bivalve molluscs in vivo and in vitro.

References

Jun 1, 1991·European Journal of Biochemistry·A J Winder, H Harris
Jun 1, 1989·Analytical Biochemistry·L M Rzepecki, J H Waite
Jan 1, 1985·Developmental and Comparative Immunology·M NakamuraT Nomura
Apr 6, 1995·Nature·A M KriegD M Klinman
Jan 1, 1997·Developmental and Comparative Immunology·R AsokanP Mullainadhan
Jul 6, 2000·The Journal of Biological Chemistry·T Nagai, S Kawabata
Feb 20, 2003·Developmental and Comparative Immunology·Zhen MengLixin Xiang
Sep 13, 2003·Fish & Shellfish Immunology·Antonio Luna-GonzálezMiguel Robles-Mungaray
Jun 24, 2004·Fish & Shellfish Immunology·T J BowdenI R Bricknell

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Citations

Aug 5, 2014·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Caterina OliveriFrancesco Marsano
Feb 24, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Giuseppe DigilioAldo Viarengo
Apr 14, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·B BouchardM Fournier
Apr 28, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Caterina CiacciLaura Canesi
Jul 26, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ming CongHuan Zhang
Oct 12, 2013·Developmental and Comparative Immunology·John T Sullivan, Joseph A Belloir
Feb 1, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jie LiuXiwu Yan

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