The impact of a short-term high-fat diet on mitochondrial respiration, reactive oxygen species production, and dynamics in oxidative and glycolytic skeletal muscles of young rats

Physiological Reports
Jean-Philippe Leduc-GaudetGilles Gouspillou

Abstract

Multiple aspects of mitochondrial function and dynamics remain poorly studied in the skeletal muscle of pediatric models in response to a short-term high-fat diet (HFD). This study investigated the impact of a short-term HFD on mitochondrial function and dynamics in the oxidative soleus (SOL) and glycolytic extensor digitorum longus (EDL) muscles in young rats. Young male Wistar rats were submitted to either HFD or normal chow (NCD) diets for 14 days. Permeabilized myofibers from SOL and EDL were prepared to assess mitochondrial respiration and reactive oxygen species (ROS) production. The expression and content of protein involved in mitochondrial metabolism and dynamics (fusion/fission) were also quantified. While no effects of HFD was observed on mitochondrial respiration when classical complex I and II substrates were used, both SOL and EDL of rats submitted to a HFD displayed higher basal and ADP-stimulated respiration rates when Malate + Palmitoyl-L-carnitine were used as substrates. HFD did not alter ROS production and markers of mitochondrial content. The expression of CPT1b was significantly increased in SOL and EDL of HFD rats. Although the expression of UCP3 was increased in SOL and EDL muscles from HFD rats, mitocho...Continue Reading

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Citations

Dec 7, 2019·Clinical Science·Chayodom ManeechoteNipon Chattipakorn
Jun 17, 2020·American Journal of Physiology. Endocrinology and Metabolism·Valerie Politis-BarberGraham P Holloway
Jul 30, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Anders E GundersenKai Zou
Aug 4, 2018·International Journal of Molecular Sciences·Brian A ParkerBenjamin T Bikman
Sep 26, 2020·International Journal of Molecular Sciences·Amanda J GendersDavid J Bishop
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Junhua YuanJing Dong
Jan 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun-Won HeoHyo-Bum Kwak

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Methods Mentioned

BETA
dissecting
electrophoresis
PCR

Software Mentioned

Data Assist
FL solutions
ImageJ
GraphPad Prism
GraphPad
Igor Pro
Primer Blast

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