PMID: 6619648Oct 1, 1983

The impact of acetazolamide on renal ammoniagenesis and gluconeogenesis

The Journal of Laboratory and Clinical Medicine
R L Tannen, B D Ross


In view of recent reports suggesting that acetazolamide (ACZ) inhibits renal ammoniagenesis and the pentose phosphate pathway, its effects on renal metabolism were investigated with rat isolated perfused kidney and isolated cortical tubules. In the isolated kidney perfused with glutamine (2 mM) and glucose (5mM), ACZ (0.1 mM) resulted in a natriuresis and bicarbonate diuresis and decreased NH3 production from 1.72 to 1.35 mumol/min/gm dry weight (p less than 0.05). NH3 production was unchanged in control perfusions in which no inhibitor was added. With kidneys from acidotic animals perfused with glutamine (2 mM) without glucose, NH3 production was unaltered by ACZ but glucose production decreased from 0.93 to 0.20 mumol/min/gm (p less than 0.01). ACZ inhibited NH3 (14.2 to 12.6 mumol/min/gm, p less than 0.01) and glucose (1.6 to 1.2 mumol/min/gm, p less than 0.05) and stimulated glutamate (2.2 to 2.9 mumol/min/gm, p less than 0.01) production by isolated tubules from normal rats incubated with 2 mM glutamine and had similar effects with acidotic animals. Glucose production from malate was also inhibited. The increase in glutamate and decrease in glucose production from glutamine, as well as malate, suggest that ACZ inhibits amm...Continue Reading

Related Concepts

Acetazolamide Sodium, (Sterile)
Glucose, (beta-D)-Isomer
Glutamic Acids
Structure of Cortex of Kidney
Renal Tubule Structure

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.