PMID: 6619648Oct 1, 1983

The impact of acetazolamide on renal ammoniagenesis and gluconeogenesis

The Journal of Laboratory and Clinical Medicine
R L Tannen, B D Ross

Abstract

In view of recent reports suggesting that acetazolamide (ACZ) inhibits renal ammoniagenesis and the pentose phosphate pathway, its effects on renal metabolism were investigated with rat isolated perfused kidney and isolated cortical tubules. In the isolated kidney perfused with glutamine (2 mM) and glucose (5mM), ACZ (0.1 mM) resulted in a natriuresis and bicarbonate diuresis and decreased NH3 production from 1.72 to 1.35 mumol/min/gm dry weight (p less than 0.05). NH3 production was unchanged in control perfusions in which no inhibitor was added. With kidneys from acidotic animals perfused with glutamine (2 mM) without glucose, NH3 production was unaltered by ACZ but glucose production decreased from 0.93 to 0.20 mumol/min/gm (p less than 0.01). ACZ inhibited NH3 (14.2 to 12.6 mumol/min/gm, p less than 0.01) and glucose (1.6 to 1.2 mumol/min/gm, p less than 0.05) and stimulated glutamate (2.2 to 2.9 mumol/min/gm, p less than 0.01) production by isolated tubules from normal rats incubated with 2 mM glutamine and had similar effects with acidotic animals. Glucose production from malate was also inhibited. The increase in glutamate and decrease in glucose production from glutamine, as well as malate, suggest that ACZ inhibits amm...Continue Reading

Related Concepts

Acetazolamide Sodium, (Sterile)
Ammonia
Diuresis
Gluconeogenesis
Glucose, (beta-D)-Isomer
Glutamic Acids
D-Glutamine
Structure of Cortex of Kidney
Renal Tubule Structure
Perfusion

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