The Impact of ackA, pta , and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12

Frontiers in Microbiology
Andrea SchützeKatja Bettenbrock

Abstract

Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by substrate level phosphorylation. Under both conditions, acetate is produced by a pathway consisting of the enzyme phosphate acetyltransferase (Pta) producing acetyl-phosphate from acetyl-coenzyme A, and of the enzyme acetate kinase (AckA) producing acetate from acetyl-phosphate, a reaction that is coupled to the production of ATP. Mutants in the AckA-Pta pathway differ from each other in the potential to produce and accumulate acetyl-phosphate. In the publication at hand, we investigated different mutants in the acetate pathway, both under aerobic as well as anaerobic conditions. While under aerobic conditions only small changes in growth rate were observed, all acetate mutants showed severe reduction in growth rate and changes in the by-product pattern during anaerobic growth. The AckA- mutant showed the most severe growth defect. The glucose uptake rate and the ATP concentration were strongly reduced in this strain....Continue Reading

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Citations

Jul 13, 2021·Journal of Bacteriology·Aiswarya Dash, Rahul Modak

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Methods Mentioned

BETA
acetylation
PCR
phosphotransferase
than

Software Mentioned

Excel
MASCOT
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