The impact of ACTH receptor knockdown on fetal and adult ovine adrenocortical cell function.

Reproductive Sciences
Yixin Su, James C Rose

Abstract

Preparing the mammalian fetus for birth requires an increase in fetal plasma glucocorticoid levels. The mechanisms facilitating this increase are not fully known. It has been shown in sheep that the prepartum elevation in fetal plasma cortisol is accompanied by increases in adrenocorticotropin receptor (ACTH-R) expression in the fetal adrenal and in the adrenal responsiveness to stimulation. To determine the significance of the upregulation in ACTH-R expression on fetal adrenal function, the authors used small interfering RNA targeted to the ovine ACTH-R to reduce receptor expression and studied responses to stimulation in ovine adrenal cells. They studied fetal cells from late gestation after responsiveness had increased. They also studied adult cells to determine if maturation would influence the impact of receptor expression suppression on responsiveness. Fetal and adult cells were obtained, dispersed, transfected with receptor-targeted small interfering RNA or scrambled small interfering RNA, and subsequently stimulated with ACTH. Cells and media were harvested for measurements of gene and protein expression and cyclic adenosine monophosphate (cAMP) and cortisol levels. The ability of ACTH to upregulate its receptor or ster...Continue Reading

References

Jan 1, 1990·Neuroendocrinology·G C AntolovichR Young
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A PenhoatJ M Saez
Aug 15, 1967·American Journal of Obstetrics and Gynecology·G C LigginsL W Holm
Mar 1, 1968·The Journal of Endocrinology·M Drost, L W Holm
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D I Buckley, J Ramachandran
Jun 1, 1996·Endocrine Reviews·D M Stocco, B J Clark
Mar 16, 2001·The Journal of Endocrinology·M FraserUNKNOWN CIHR Group in Fetal and Neonatal Health and Development
Dec 10, 2002·Current Opinion in Chemical Biology·Reuven Agami
Jan 7, 2004·Journal of the Society for Gynecologic Investigation·J J WangJ C Rose
Jul 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Marc SlawikFelix Beuschlein
Mar 22, 2005·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·P R Manna, D M Stocco
Apr 2, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nancy K ValegoJames C Rose
Nov 8, 2005·Trends in Endocrinology and Metabolism : TEM·Adrian J L ClarkAngela Huebner
Feb 16, 2006·American Journal of Physiology. Endocrinology and Metabolism·Luke C CareyJames C Rose
Jul 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·George L Sen, Helen M Blau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.