The Impact of BPI Expression on Escherichia coli F18 Infection in Porcine Kidney Cells

Animals : an Open Access Journal From MDPI
Jian JinWenbin Bao

Abstract

The efficacy and regulatory activity of bactericidal/permeability-increasing protein (BPI) as a mediator of Escherichia coli (E. coli) F18 resistance remains to be defined. In the present study, we evaluated lipopolysaccharide (LPS)-induced changes in BPI gene expression in porcine kidney (PK15) cells in response to E. coli F18 exposure. We additionally generated PK15 cells that overexpressed BPI to assess the impact of this gene on Toll-like receptor 4 (TLR4) signaling and glycosphingolipid biosynthesis-related genes. Through these analyses, we found that BPI expression rose significantly following LPS exposure in response to E. coli F18ac stimulation (p < 0.01). Colony count assays and qPCR analyses revealed that E. coli F18 adherence to PK15 cells was markedly suppressed following BPI overexpression (p < 0.01). BPI overexpression had no significant effect on the mRNA-level expression of genes associated with glycosphingolipid biosynthesis or TLR4 signaling. BPI overexpression suppressed the LPS-induced TLR4 signaling pathway-related expression of proinflammatory cytokines (IFN-α, IFN-β, MIP-1α, MIP-1β and IL-6). Overall, our study serves as an overview of the association between BPI and resistance to E. coli F18 at the cellu...Continue Reading

References

Jan 1, 1996·Critical Reviews in Microbiology·A PrestonM A Apicella
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 17, 2002·Annals of Medicine·Daisy W J van der SchaftArjan W Griffioen
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Nov 12, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Geraldine CannySean P Colgan
Oct 3, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michiko YamagataGeorge L King
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Deborah J LenschowHerbert Whiting Virgin
Feb 23, 2010·Thrombosis and Haemostasis·Eduardo EsteveJosé Manuel Fernández-Real
Feb 14, 2012·Current Opinion in Virology·John W Schoggins, Charles M Rice
May 11, 2013·Genetics and Molecular Research : GMR·J ZhuW B Bao
Aug 5, 2014·Expert Review of Clinical Immunology·Saleem BashaMichael Pichichero
Jan 4, 2018·International Journal of Chronic Obstructive Pulmonary Disease·Elise G De SmetKen R Bracke
May 3, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Michael S Little, Matthew R Redinbo
Oct 26, 2018·International Journal of Molecular Sciences·Zhengchang WuWenbin Bao

❮ Previous
Next ❯

Citations

Mar 7, 2021·Animals : an Open Access Journal From MDPI·Andrea BonettiEster Grilli

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked
Assay
fluorescence microscopy
ELISA
electrophoresis
co-immunoprecipitation
gene knockout

Software Mentioned

ImageJ
Primer Premier
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.