The Impact of Cell-Expansion and Inflammation on The Immune-Biology of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

Journal of Clinical Medicine
Karolien BuylMehdi Najar

Abstract

Background: As a cell-based therapeutic, AT-MSCs need to create an immuno-reparativeenvironment appropriate for tissue repair. In the presence of injury, MSCs may have to proliferate and face inflammation. Clinical application requires repeated administrations of a high number of cellswith a well-established immune profile. Methods: We have established an immuno-comparative screening by determining the expression of 28 molecules implicated in immune regulation. This screening was performed during cell-expansion and inflammatory priming of AT-MSCs. Results: Our study confirms that AT-MSCs are highly expandable and sensitive to inflammation. Both conditions have substantially modulated the expression of a panel of immunological marker. Specifically, CD34 expression was substantially decreased upon cell-passaging. HLA-ABC, CD40 CD54, CD106, CD274 and CD112 were significantly increased by inflammation. In vitro cell-expansion also significantly altered the expression profile of HLA-DR, CD40, CD62L, CD106, CD166, HLA-G, CD200, HO-1, CD155 and ULBP-3. Conclusion: This study points out the response and characteristics of MSCs following expansion and inflammatory priming. It will strength our knowledge about the molecular mechanisms th...Continue Reading

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Citations

May 24, 2020·Journal of Clinical Medicine·Makram MerimiMehdi Najar
Jan 7, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Makram MerimiMehdi Najar
Jan 29, 2021·World Journal of Stem Cells·Mehdi NajarHassan Fahmi

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Methods Mentioned

BETA
flow cytometry
bone marrow aspiration

Software Mentioned

Graph
Prism
- Pad

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