The Impact of Extended Typing On Red Blood Cell Alloimmunization in Transfused Patients

Open Access Macedonian Journal of Medical Sciences
T Makarovska-BojadzievaMilenka Blagoevska

Abstract

Red blood cell (RBC) alloimmunization is still an actual problem in our transfusion practice. In 2011, in addition to the regular ABO/D blood group typing, phenotyping for Rh (C, c, E, e) and Kell antigens was introduced for blood donors and patients undergoing blood transfusion. Our aim was to evaluate the impact of the extended RBC typing and donor/recipient matching on the incidence of RBC alloimmunization. A retrospective comparative study was conducted by reviewing RBC request records for about 36,000 patients transfused with RBC in the period from 2013 to 2015 in comparison to the similar study conducted on 47,000 transfused patients in the period from 2005 to 2008. Pre-transfusion serologic testing data were retrieved for analysis. Blood samples with positive antibody screening and positive cross-match were further subjected to antibody identification. All the tests were performed using column agglutination technique (CAT) with ID-cards and reagents from DiaMed in both studies. Irregular RBC alloantibodies were detected in 116 (0.32%) out of 36,000 transfused patients. Multiple transfusions (15.8 units/patient) were given to 450 patients from which 79 (17.5%) had RBC allontibodies. The incidence of RBC alloimmunisation i...Continue Reading

Citations

May 31, 2018·Transfusion Medicine·E G da Cunha GomesJ F N Neto

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping

Software Mentioned

BLOODchip

Related Concepts

Related Feeds

Blood Group Incompatibility

Blood group incompatibility between donor and recipient has been associated with poor transplant outcomes. Discover the latest research on blood group icompatibility here.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.