The impact of Helicobacter pylori infection, eradication therapy and probiotic supplementation on gut microenvironment homeostasis: An open-label, randomized clinical trial

EBioMedicine
Luyi ChenShujie Chen

Abstract

Helicobacter pylori (H. pylori) infection is associated with remodeling of gastric microbiota. However, comprehensive analyses of the impact of H. pylori infection, eradication therapy and probiotic supplementation on gut microbiota are still lacking. We aimed to provide evidence for clinical decision making. Seventy H. pylori-positive and 35 H. pylori-negative patients (group C) were enrolled. H. pylori-positive patients were randomly assigned to group A (14-day bismuth-containing quadruple therapy) and group B (quadruple therapy supplemented with Clostridium butyricum). Stool samples of group A and B were collected on day 0, 14 and 56 while stool samples of group C were collected on day 0. Gut microbiota was investigated by 16S rRNA sequencing. The Sobs index (richness estimator) was significantly higher in H. pylori-positive samples than H. pylori-negative samples (p < .05). Several metabolic pathways were more abundant in H. pylori-positive communities while some disease-associated pathways had higher potential in H. pylori-negative community through KEGG pathway analysis. Abundances of most butyrate-producing bacteria significantly decreased, while several detrimental bacteria increased after eradication therapy. Probiotic...Continue Reading

Citations

May 23, 2019·Journal of Gastroenterology and Hepatology·Ping-I HsuKuo-Wang Tsai
Feb 11, 2020·Digestive Diseases and Sciences·Lars Engstrand, David Y Graham
Jan 28, 2020·Journal of Gastroenterology and Hepatology·Jyh-Ming LiouMing-Shiang Wu
Feb 25, 2020·Movement Disorders : Official Journal of the Movement Disorder Society·Mohamed ElfilSara M Schaefer
Sep 9, 2020·Helicobacter·Andrés Suárez-JaramilloPaul A Cárdenas
Mar 7, 2020·Anaesthesia and Intensive Care·Kwok M HoEdward Litton
Apr 17, 2020·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Airu LiuYiqi Du
Sep 6, 2019·Helicobacter·Anthony O'ConnorColm O'Morain
Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·Lu YangZhongtao Gai
Oct 3, 2020·Gut·Jyh-Ming LiouUNKNOWN Asian Pacific Alliance on Helicobacter and Microbiota (APAHAM)
Jun 19, 2019·PloS One·Nihar Ranjan DashMohammad Tahseen Al Bataineh
Aug 22, 2020·World Journal of Gastroenterology : WJG·Maria Luísa Cordeiro SantosFabrício Freire de Melo
May 4, 2021·Gut Microbes·Chieh-Chang ChenMing-Shiang Wu
May 19, 2021·Nature Reviews. Gastroenterology & Hepatology·Evariste Tshibangu-Kabamba, Yoshio Yamaoka
Jul 6, 2021·Frontiers in Endocrinology·Gracia M Martin-NuñezFrancisco J Tinahones
Sep 25, 2021·BMC Microbiology·Seyedeh Zahra Bakhti, Saeid Latifi-Navid
Sep 28, 2021·Critical Reviews in Microbiology·Cláudia SousaLuís D R Melo
Dec 21, 2021·International Journal of Antimicrobial Agents·Kristien Nel Van ZylMae Newton-Foot

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPSS
Cladogram
PICRUSt ( Phylogenetic Investigation of Communities by Reconst...
Trimmomatic
LEfSe
Mothur
Quantitative Insights Into Microbial Ecology ( QIIME
FLASH
UPARSE

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