The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis.

Investigative Ophthalmology & Visual Science
Connie TamSuzanne M J Fleiszig

Abstract

Contact lens wear predisposes to Pseudomonas aeruginosa keratitis, but the mechanisms involved remain unclear. An in vivo model was used to study lens inoculation conditions enabling disease. Custom-made hydrogel contact lenses were fitted to rats after incubation in P. aeruginosa approximately 10(11) cfu/mL (3 hours) or approximately 10(3) cfu/mL (24 hours). Another group was inadvertently inoculated with a suction pen previously used with high inocula, but rinsed in ethanol and stored dry (6 months). Some corneas were tissue paper-blotted to cause fluorescein staining before lens fitting. Contralateral eyes were untreated. Twenty-four hours after disease detection, lenses were transferred to naive rats or examined by confocal microscopy before homogenization to quantify viable bacteria. After lens removal, corneas were washed to collect nonadherent bacteria and were analyzed by immunohistochemistry. All eyes challenged with unworn contaminated lenses developed keratitis after approximately 7 to 10 days. Disease delay and severity were unaffected by inoculum parameters or tissue blotting but occurred sooner with lenses transferred from infected eyes ( approximately 2 days). Worn lenses and corneal washes contained infecting ba...Continue Reading

Citations

Aug 8, 2012·Eye & Contact Lens·Mark D P WillcoxAjay K Vijay
Dec 26, 2012·Eye & Contact Lens·David J Evans, Suzanne M J Fleiszig
Dec 26, 2012·Eye & Contact Lens·Mark D P Willcox
Dec 1, 2010·Infection and Immunity·Danielle K AugustinSuzanne M J Fleiszig
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Mary E Marquart
Mar 25, 2014·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Danielle M RobertsonNancy McNamara
Mar 26, 2015·Pathogens·Paulo J M BispoMichael S Gilmore
Nov 6, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Merle FernandesNagendra Kumar
Apr 16, 2013·The Ocular Surface·Srihari NarayananAlison M McDermott
Mar 30, 2012·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Victoria B TranC J Radke
Oct 12, 2012·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Nathan Efron
Apr 23, 2013·American Journal of Ophthalmology·David J Evans, Suzanne M J Fleiszig
Jan 31, 2013·International Reviews of Immunology·Eric PearlmanArne Rietsch
Oct 26, 2016·Cellular Microbiology·Nichole Orench-Rivera, Meta J Kuehn
May 8, 2015·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Jerome OzkanMark D P Willcox
Nov 23, 2017·BMC Ophthalmology·Dong Ju KimChoul Yong Park
Nov 22, 2019·Journal of Medical Microbiology·Yasmin HilliamCraig Winstanley
Feb 13, 2020·Journal of Cataract and Refractive Surgery·Andrew L SorensonDavid F Chang
Jun 20, 2019·Frontiers in Cellular and Infection Microbiology·Manon Ruffin, Emmanuelle Brochiero
Jan 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan SunEric Pearlman
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan SunEric Pearlman
Nov 23, 2019·Progress in Retinal and Eye Research·Suzanne M J FleiszigDavid J Evans
Jun 8, 2021·Experimental Eye Research·Lawson Ung, James Chodosh
Jun 29, 2021·Virulence·Charles D MorinGee W Lau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.