The impact of long-term exposure to space environment on adult mammalian organisms: a study on mouse thyroid and testis.

PloS One
Maria Angela MasiniFrancesco Saverio Ambesi-Impiombato

Abstract

Hormonal changes in humans during spaceflight have been demonstrated but the underlying mechanisms are still unknown. To clarify this point thyroid and testis/epididymis, both regulated by anterior pituitary gland, have been analyzed on long-term space-exposed male C57BL/10 mice, either wild type or pleiotrophin transgenic, overexpressing osteoblast stimulating factor-1. Glands were submitted to morphological and functional analysis.In thyroids, volumetric ratios between thyrocytes and colloid were measured. cAMP production in 10(-7)M and 10(-8)M thyrotropin-treated samples was studied. Thyrotropin receptor and caveolin-1 were quantitized by immunoblotting and localized by immunofluorescence. In space-exposed animals, both basal and thyrotropin-stimulated cAMP production were always higher. Also, the structure of thyroid follicles appeared more organized, while thyrotropin receptor and caveolin-1 were overexpressed. Unlike the control samples, in the space samples thyrotropin receptor and caveolin-1 were both observed at the intracellular junctions, suggesting their interaction in specific cell membrane microdomains.In testes, immunofluorescent reaction for 3β- steroid dehydrogenase was performed and the relative expressions of...Continue Reading

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Citations

May 29, 2014·PloS One·Elisabetta AlbiFrancesco Saverio Ambesi-Impiombato
Jun 3, 2014·Cell Communication and Signaling : CCS·Elisabeth WarnkeDaniela Grimm
Mar 7, 2014·Tissue Engineering. Part B, Reviews·Daniela GrimmJohann Bauer
Jul 24, 2014·Journal of Tissue Engineering and Regenerative Medicine·Alessandra Ruggiu, Ranieri Cancedda
Oct 21, 2014·BioMed Research International·Elisabetta AlbiFrancesco Saverio Ambesi-Impiombato
May 5, 2017·International Journal of Molecular Sciences·Elisabetta AlbiFrancesco Curcio
Oct 16, 2019·Nature Reviews. Endocrinology·Birendra Mishra, Ulrike Luderer
Apr 17, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pingping LiQing Ge
May 30, 2019·International Journal of Molecular Sciences·Marcus KrügerDaniela Grimm
May 11, 2021·Frontiers in Bioengineering and Biotechnology·Fabio CostaElisabetta Albi
Oct 13, 2021·Environmental Health and Preventive Medicine·Fenju QinGuangming Zhou

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Methods Mentioned

BETA
transgenic
MDS
ISS
electrophoresis
PCR

Software Mentioned

Scion Image
ImageFocus
Genex
Scion Image programme
- Gene Expression MacroTM
Biorad

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