The impact of mechanical ventilation on immature airway smooth muscle: functional, structural, histological, and molecular correlates

Biology of the Neonate
Aaron B CullenT H Shaffer

Abstract

Preterm infants exposed to mechanical ventilation often develop airway dysfunction and bronchopulmonary dysplasia. The mechanisms of mechanical ventilation-induced airway injury are currently unknown. This study correlates the age-related effects of mechanical ventilation on airway function with structural alterations at the tissue, cellular, ultrastructural, and molecular levels. Mechanically ventilated and nonventilated tracheal rings were obtained from premature and newborn lambs. In tissue baths, the passive and active length-tension relationships and dose-response characteristics of the tracheal rings were determined. Fixed tracheal rings were digested and the resulting isolated smooth muscle cells measured. Rings were analyzed by light and electron microscopy. Additionally, protein was extracted from the tracheal smooth muscle and myosin heavy chain isoforms were separated by SDS-polyacrylamide gel electrophoresis and analyzed by densitometry. Mechanical ventilation resulted in a significant decrease of both the slope of the passive length-stress relationship and of maximal force generation, with both effects being most pronounced in the newborn age group. These age-related functional alterations correlated with a decreas...Continue Reading

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Citations

Feb 10, 2010·Annals of Biomedical Engineering·M MalvèM Doblaré
Feb 19, 2013·Clinical and Experimental Pharmacology & Physiology·Megan O'ReillyRichard Harding
Aug 10, 2011·Journal of Biomechanical Engineering·M MalvèM Doblaré
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Oct 10, 2009·Pediatric Research·Noah H HillmanAlan H Jobe
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Apr 25, 2008·Neonatal Network : NN·Jeanne Ray WiedemannKaren Duran
Sep 1, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Megan O'ReillyFoula Sozo

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