The impact of media composition on production of flavonoids in agitated shoot cultures of the three Hypericum perforatum L. cultivars 'Elixir,' 'Helos,' and 'Topas'

In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association
Inga KwiecieńHalina Ekiert

Abstract

The aims of the study were to evaluate the effect of media composition on the growth potential and morphology of the in vitro cultured biomass of three cultivars of Hypericum perforatum, and on the production of flavonoids. Agitated shoot cultures were maintained in parallel on Linsmaier and Skoog (LS) and Murashige and Skoog (MS) media supplemented with 0.1-3.0 mg L-1 of α-naphthaleneacetic acid and 6-benzylaminopurine. Methanolic extracts from the biomass collected after 3-wk growth cycles were analyzed quantitatively, for 21 flavonoids using high performance liquid chromatography. Three aglycones (kaempferol, luteolin, and quercetin) and three glycosides of quercetin (hyperoside, quercitrin, and rutoside) were detected in all of the extracts. The total amounts of the estimated compounds increased from 1.18- to 21.66-fold on LS media variants and from 1.52- to 17.34-fold on MS media variants. The main metabolite was quercetin (max. 210.55 mg 100 g-1 dry weight [DW]). The maximum total amounts of all compounds in the biomass of 'Elixir,' 'Helos,' and 'Topas' were 328.53, 255.70, and 166.58 mg 100 g-1 DW, respectively. The shoots of all cultivars cultivated on the LS and MS media containing low levels of plant growth regulators...Continue Reading

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Citations

Mar 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cengiz SarikurkcuMichał Tomczyk
Apr 25, 2018·Applied Microbiology and Biotechnology·Agnieszka SzopaHalina Ekiert
Dec 17, 2018·Molecular Biology Reports·Gabriela Carmona-CastroIrene Perea-Arango

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Software Mentioned

Helos
TIBCO®
Elixir
Topas
[UNK]

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