PMID: 19141957Jan 15, 2009Paper

The impact of micafungin on the cellular integrity of mg63 osteoblastlike cells in vitro a preliminary study

Biomedical Sciences Instrumentation
P I SealyHamed A Benghuzzi

Abstract

Fungal osteomyelitis is a rare and possibly life-threatening condition. Factors that increase the risk of bone infection include the use of indwelling catheters, immunosuppressive agents (cyclosporine) in organ transplantation, aggressive anticancer chemotherapy, total parenteral nutrition, and broad spectrum antibiotics; immunocompromised hosts (human immunodeficiency virus) and individuals who have suffered burns, have diabetes mellitus or have undergone abdominal surgery. Treatment involves the use of intravenously administered antifungal agents, such as micafungin (MFG), initially, followed by long term oral suppressive therapy. The purpose of this study was to assess the impact of MFG at low (0.128 microg/mL), medium (64 microg/mL) and high (125 microg/mL) concentrations on MG-63 osteoblast-like cells (MOCs) on cell growth and viability. Apoptosis, glutathione and malondialdehyde assays, hematoxylin and eosin stains, and the proliferation rate were used to characterize MOCs (1 x 104 cells/well) for growth and viability after exposure to low, medium and high concentrations of MFG at 24, 48 and 72 hours in tissue culture. Micafungin at 0.128 and 64 microg/mL had a stimulatory effect on bone cell proliferation as early as 24 ...Continue Reading

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis