The Impact of Oncogenic EGFRvIII on the Proteome of Extracellular Vesicles Released from Glioblastoma Cells.

Molecular & Cellular Proteomics : MCP
Dongsic ChoiJanusz Rak

Abstract

Glioblastoma multiforme (GBM) is a highly aggressive and heterogeneous form of primary brain tumors, driven by a complex repertoire of oncogenic alterations, including the constitutively active epidermal growth factor receptor (EGFRvIII). EGFRvIII impacts both cell-intrinsic and non-cell autonomous aspects of GBM progression, including cell invasion, angiogenesis and modulation of the tumor microenvironment. This is, at least in part, attributable to the release and intercellular trafficking of extracellular vesicles (EVs), heterogeneous membrane structures containing multiple bioactive macromolecules. Here we analyzed the impact of EGFRvIII on the profile of glioma EVs using isogenic tumor cell lines, in which this oncogene exhibits a strong transforming activity. We observed that EGFRvIII expression alters the expression of EV-regulating genes (vesiculome) and EV properties, including their protein composition. Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRvIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. Among them, we validated the association of pro-invasive proteins (CD44, BSG, CD151) with EVs...Continue Reading

References

Dec 26, 2001·Molecular and Cellular Neurosciences·H WernerK A Nave
Oct 7, 2004·Cancer Research·Hiroko OhgakiPaul Kleihues
Jul 19, 2005·Blood Cells, Molecules & Diseases·K LaulagnierM Record
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Julia Y LjubimovaKeith L Black
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Nov 3, 2007·Genes & Development·Frank B FurnariWebster K Cavenee
Sep 6, 2008·Science·D Williams ParsonsKenneth W Kinzler
Sep 6, 2008·Nature·UNKNOWN Cancer Genome Atlas Research Network
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Khalid Al-NedawiJanusz Rak
Jul 2, 2009·Molecular & Cellular Proteomics : MCP·Oscar AlcazarMaria E Marin-Castano
Jun 10, 2010·Biochemical and Biophysical Research Communications·Hwan Myung LeeYong Song Gho
Sep 15, 2010·The Journal of Cell Biology·Arthit ChairoungduaMichael J Caplan
Nov 26, 2010·Cancer Research·An HendrixOlivier De Wever
Dec 22, 2010·Cell Adhesion & Migration·Soo-Young KimHyun Kim
Jan 22, 2011·Nature Reviews. Molecular Cell Biology·Roi Avraham, Yosef Yarden
Mar 30, 2011·Glia·Nikki A CharlesHelmut Kettenmann
Apr 12, 2011·Cancer Research·Joshua L HoodSamuel A Wickline
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jingjing HuSteven L Gonias
Nov 1, 2011·The International Journal of Biochemistry & Cell Biology·Sylwia Owczarek, Vladimir Berezin
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
Nov 20, 2012·Molecular & Cellular Proteomics : MCP·Michelle Demory BecklerRobert J Coffey
Dec 12, 2012·Molecular & Cellular Proteomics : MCP·Bow J TauroRichard J Simpson
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Paulina KucharzewskaMattias Belting
Apr 30, 2013·Current Biology : CB·Christopher R WoodJoel L Rosenbaum
Aug 3, 2013·Neoplasia : an International Journal for Oncology Research·Wei MuMargot Zöller
Aug 7, 2013·Nature Genetics·Veronique FrattiniAntonio Iavarone
Sep 7, 2013·Journal of Extracellular Vesicles·Dae-Kyum KimYong Song Gho

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Citations

Dec 19, 2018·Proteomics·Dongsic ChoiJanusz Rak
Oct 27, 2018·Journal of Cellular Biochemistry·Keqiu JiangLiming Wang
Apr 6, 2020·Journal of Neurochemistry·Vanesa S MatteraJorge D Correale
May 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Siyuan ChenShenglin Huang
Jul 9, 2020·International Journal of Molecular Sciences·Susannah HallalKimberley Louise Alexander-Kaufman
Jul 19, 2020·Reviews in the Neurosciences·Ge WangMujun Liu
Aug 5, 2020·Cell Communication and Signaling : CCS·Amir B GhaemmaghamiMichael R Hamblin
Apr 13, 2019·Annual Review of Genomics and Human Genetics·Michael W Graner
Aug 24, 2019·Journal of Hematology & Oncology·Xinyu TianShengjun Wang
Mar 7, 2020·Metabolites·Miroslava Čuperlović-CulfJagdeep K Sandhu
Dec 19, 2018·Neuro-oncology·Kyle FraserRalph Weissleder
Feb 11, 2020·Frontiers in Pharmacology·Shilpa ChennakrishnaiahJanusz Rak
Jul 11, 2019·American Journal of Physiology. Cell Physiology·Marie LafitteSerge Roche
Dec 24, 2019·Critical Reviews in Clinical Laboratory Sciences·Clarissa A WhiteheadStanley S Stylli
Feb 8, 2020·Science·Raghu Kalluri, Valerie S LeBleu
Nov 19, 2020·Molecular Oncology·Antonia Schubert, Michael Boutros
Feb 7, 2021·Annals of Oncology : Official Journal of the European Society for Medical Oncology·W YuJ K Skog
Apr 3, 2021·Advanced Drug Delivery Reviews·Sander A A KooijmansRaymond M Schiffelers
Apr 7, 2021·Cellular and Molecular Life Sciences : CMLS·Ernest T ChiveroGuoku Hu
Jun 2, 2021·Nano Letters·Imman I HosseiniSara Mahshid
Jun 18, 2021·Journal of Extracellular Vesicles·Dongsic ChoiJanusz Rak
Jun 15, 2021·FASEB BioAdvances·James W ClancyCrislyn D'Souza-Schorey
Dec 28, 2019·Analytical Chemistry·Carolina Fernández-CostaJohn R Yates

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