The impact of oregano (Origanum heracleoticum) essential oil and carvacrol on virulence gene transcription by Escherichia coli O157:H7

FEMS Microbiology Letters
Hasika MithVéronique Delcenserie

Abstract

The aim of the current study was to determine, via reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis, the effect of oregano essential oil (Origanum heracleoticum) and carvacrol, its major component, on the expression of virulence-associated genes in enterohaemorrhagic Escherichia coli (EHEC) O157:H7 ATCC strain 35150. Both oregano oil and carvacrol demonstrated their efficacy firstly, by inhibiting the transcription of the ler gene involved in upregulation of the LEE2, LEE3 and LEE4 promoters and of attaching and effacing lesions and secondly by decreasing both Shiga toxin and fliC genes expression. In addition, a decrease in luxS gene transcription involved in quorum sensing was observed. These results were dose dependent and showed a specific effect of O. heracleoticum and carvacrol in downregulating the expression of virulence genes in EHEC O157:H7. These findings suggest that oregano oil and carvacrol have the potential to mitigate the adverse health effects caused by virulence gene expression in EHEC O157:H7, through the use of these substances as natural antibacterial additives in foods or as an alternative to antibiotics.

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Citations

Nov 20, 2015·The Journal of General and Applied Microbiology·Satoshi OguriHiroyuki Yamaguchi
Jan 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Javad Sharifi-RadMarcello Iriti
Aug 16, 2016·Applied and Environmental Microbiology·Judit K KovácsGyörgy Schneider
Oct 3, 2018·Foodborne Pathogens and Disease·Alexandros Ch StratakosNicolae Corcionivoschi
Jul 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Teresa Della PepaLucia Caputo
Mar 27, 2020·BMC Complementary Medicine and Therapies·Shuzhen XiaoYing Zhang
Dec 18, 2020·Comprehensive Reviews in Food Science and Food Safety·Dan ZhangHarold Corke

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