The impact of phospholipids and phospholipid removal on bioanalytical method performance

Biomedical Chromatography : BMC
Jennifer Carmical, Stacy Brown

Abstract

Phospholipids (PLs) are a component of cellmembranes, biological fluids and tissues. These compounds are problematic for the bioanalytical chemist, especially when PLs are not the analytes of interest. PL interference with bioanalysis highly impacts reverse-phase chromatographic methods coupled with mass spectrometric detection. Phospholipids are strongly retained on hydrophobic columns, and can cause significant ionization suppression in the mass spectrometer, as they outcompete analyte molecules for ionization. Strategies for improving analyte detection in the presence of PLs are reviewed, including in-analysis modifications and sample preparation strategies. Removal of interfering PLs prior to analysis seems to be most effective atmoderating thematrix effects fromthese endogenous cellular components, and has the potential to simplify chromatography and improve column lifetime. Products targeted at PL removal for sample pre-treatment, as well as products that combine multiplemodes of sample preparation (i.e. Hybrid SPE), show significant promise inmediating the effect on PL interference in bioanalysis.

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Citations

Jul 20, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kristine Skoglund AskAstrid Gjelstad
Dec 14, 2017·Biomedical Chromatography : BMC·Michael G Bartlett
Nov 17, 2017·Biomedical Chromatography : BMC·Andrei MedvedoviciVictor David
Jul 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manuela CortesePiera Di Di Martino
Jan 11, 2018·Analytical and Bioanalytical Chemistry·Giorgia La BarberaAldo Laganà
Jun 17, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Raúl González-DomínguezCristina Andrés-Lacueva
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Jul 5, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nikunj TannaRobert S Plumb
Oct 5, 2019·Environmental Science & Technology·Hanna M DuszaJuliette Legler

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