The impact of polyunsaturated fatty acids on DNA methylation and expression of DNMTs in human colorectal cancer cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Mostafa Moradi Sarabi, Fakhraddin Naghibalhossaini

Abstract

Growing evidence suggests a role of polyunsaturated fatty acids (PUFA) in the prevention of various types of malignancy, including colorectal cancer (CRC). No published studies have yet examined the direct effect of PUFA treatment on DNA methylation in CRC cells. In this study, 5 human CRC cells were treated with 100 μM DHA, EPA, and LA for 6 days and changes in their global- and gene-specific DNA methylation status as well as expression of DNA methyl transferases (DNMT) were investigated. Cell-type specific differences in DNA methylation and expression of DNMTs were observed in PUFA-treated cells. DHA and EPA treatment induced global hypermethylation in HT29/219 and HCT116 cells, but reduced methylation in Caco2 cells (p < 0.05). Among 10 tumor related genes tested in 5 CRC cell lines, DHA and EPA induced promoter demethylation of Cox2 in HT29/219, p14 and PPARγ in HCT116, and ECAD in SW742 cells. Cell-type specific differences in expression of DNMT1, DNMT3a, and 3b genes were also observed between PUFA-treated and control cells (p < 0.05). Overall, treatment of PUFAs coordinately induced the expression of DNMTs in HT29/219, but suppressed in other 4 cell lines investigated in this study.

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Citations

Feb 12, 2020·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Fatemeh ZalMostafa Moradi Sarabi
Jan 9, 2020·World Journal of Clinical Cases·Mostafa Moradi SarabiEbrahim Eftekhar
Aug 20, 2019·Journal of Physiology and Biochemistry·Mirian SamblasFermín I Milagro
Oct 3, 2020·World Journal of Gastrointestinal Oncology·Amirsaeed Sabeti AghabozorgiEbrahim Eftekhar
Jul 27, 2021·Environmental Science and Pollution Research International·Nasreddine El OmariAbdelhakim Bouyahya
Aug 24, 2021·Frontiers in Nutrition·Maja MiloševićVesna Vučić

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