The impact of sequence divergence and DNA mismatch repair on homeologous recombination in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Liangliang LiFrancois J Belzile

Abstract

We examined the effects of substrate divergence and DNA mismatch repair (MMR) on recombination in Arabidopsis thaliana. Relative to the frequency observed in plants with a homologous construct (0% divergence), recombination was decreased 4.1-, 9.6-, 11.7- or 20.3-fold, respectively, in lines with constructs containing 0.5%, 2%, 4% or 9% divergence between the recombination substrates. To evaluate the contribution of the MMR system in this decrease, 12 independent reporter lines (two or three lines per reporter construct) were crossed to an AtMSH2 T-DNA insertional mutant. We examined the recombination frequency in progeny homozygous for a reporter T-DNA and homozygous either for the wild type or the mutant allele of AtMSH2. The loss of MMR activity led to a two- to ninefold increase in homeologous recombination and the size of the increase did not seem to correlate with the amount of divergence. Inversely, complementation of the insertional mutant with a wild-type cDNA of AtMSH2 reduced recombination. Our results demonstrate clearly that sequence divergence can dramatically reduce the recombination frequency in plants and that the MMR system plays a part in this decrease.

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O P DasJ Messing
Jan 1, 1997·Molecular and Cellular Biology·M T NegrittoA M Bailis
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·A DattaS Jinks-Robertson
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M VulićM Radman
Oct 12, 1999·Molecular & General Genetics : MGG·J AdéM P Doutriaux
Nov 28, 2000·Annual Review of Genetics·B D Harfe, S Jinks-Robertson
Oct 7, 2003·Annual Review of Microbiology·Mark J Schofield, Peggy Hsieh
Oct 7, 2004·Cytogenetic and Genome Research·J A SurteesE Alani
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Liangliang LiFrançois Belzile
Dec 18, 2004·Plant Molecular Biology·Abdourahamane H AlouFrancois J Belzile
Feb 26, 2005·Nucleic Acids Research·Melissa A CalmannM G Marinus
Jun 1, 1996·Genome Génome / Conseil National De Recherches Canada·T LiharskaM Koornneef

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Citations

Aug 27, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sheh May TamRoger T Chetelat
Aug 6, 2010·Molecular Biotechnology·Rodrigo L GomezClaudia P Spampinato
Nov 6, 2008·Annual Review of Genetics·Jeff J DoyleJonathan F Wendel
Aug 30, 2008·BMC Plant Biology·Alice NavrátilováJirí Macas
Jun 13, 2012·Journal of Biomedicine & Biotechnology·Claudia P Spampinato, Diego F Gomez-Casati
Jul 23, 2009·Mutation Research·Claudia P SpampinatoLuciana D Lario
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Eric DionFrançois Belzile
Dec 4, 2014·Journal of Experimental Botany·Luciana Daniela LarioClaudia Patricia Spampinato
Feb 12, 2014·Plant Signaling & Behavior·Zoë MigicovskyIgor Kovalchuk
Feb 13, 2007·Current Opinion in Plant Biology·Jin LiPatrick S Schnable
Sep 17, 2020·The EMBO Journal·Alexander R BlackwellIan R Henderson
Jun 24, 2010·Molecular Biology Reports·Celina GallesClaudia P Spampinato
Nov 12, 2015·Frontiers in Plant Science·Vasilissa Manova, Damian Gruszka
Mar 10, 2008·Sensors·Igor Kovalchuk, Olga Kovalchuk
Mar 21, 2020·Frontiers in Plant Science·Meng JiangHairui Cui
Sep 21, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Ivone Un San LeongDonald R Love
Feb 7, 2019·Frontiers in Plant Science·Elena Rakosy-TicanAdriana Aurori
Dec 12, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Marion DelameDidier Merdinoglu
Oct 8, 2020·Frontiers in Genetics·Prabha VermaVineet Gaur
Jul 10, 2021·Frontiers in Genetics·Aamir RainaRais A Ganai
Jul 13, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Daniel L JeffriesJohn R Pannell

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