The impact of standard chemotherapy on miRNA signature in plasma in AML patients

Leukemia Research
Linda KoutovaMarie Korabecna

Abstract

In our pilot study, we used plasma samples as liquid biopsy to search for miRNA signatures in patients with acute myeloid leukemia (AML) at diagnosis and in remission achieved after standard chemotherapy before planned transplantation. We examined 10 plasma samples from healthy volunteers and 8 paired samples from patients with AML at diagnosis and in remission using TaqMan MicroRNA Arrays. The results were validated using single-target qPCR reactions run in triplicates. We selected 6 miRNAs with expressions significantly sensitive to therapy: miR-199b-5p, miR-301b, miR-326, miR-361-5p, miR-625 and miR-655. All selected miRNAs were not or very weakly expressed in healthy individuals. They were abundant in plasma in patients at diagnosis but their levels decreased after chemotherapy. We detected a therapy sensitive miRNA signature in plasma of patients with AML.

References

Aug 4, 2012·PloS One·Kai WangDavid J Galas
Feb 9, 2013·Journal of Translational Medicine·Hussein Fayyad-KazanBassam Badran
Jun 18, 2014·The Journal of Clinical Endocrinology and Metabolism·Donato SantovitoFrancesco Cipollone

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Citations

Aug 6, 2018·Journal of Cellular Physiology·Golamreza JadideslamAlireza Khabbazi
Feb 15, 2018·Frontiers in Oncology·Stephanie Rebecca SetijonoSu Jung Song
May 10, 2019·Journal of Cellular Physiology·Golamreza JadideslamAlireza Kabbazi

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