The impact of storage temperature and sperm number on the fertility of liquid-stored bull semen

Reproduction, Fertility, and Development
Craig MurphyS Fair

Abstract

In Ireland, liquid bull semen is stored at unregulated ambient temperatures, typically at 5×106 spermatozoa per dose, and inseminated within 2.5 days of collection. In Experiment 1, the effect of storage temperature (5, 15, 22, 32°C and fluctuations (Flux) between these temperatures) on progressive motility, viability, acrosomal status, DNA fragmentation and osmotic resistance was assessed. In Experiment 2, the field fertility of liquid semen at 5, 4 and 3×106 spermatozoa per dose, up to Day 2 after collection, was assessed in comparison to frozen-thawed semen at 20×106 spermatozoa per dose (n=35328 inseminations). In Experiment 1, storage at 15°C resulted in the highest progressive motility (PP6 spermatozoa per dose on Day 2 of storage was reduced in comparison to frozen-thawed semen (P<0.01). In conclusion, liquid semen is versatile between storage temperatures of 5 and 22°C, but demonstrates reduced fertility on Day 2 of storage at lower sperm numbers in comparison to frozen-thawed semen.

References

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Citations

May 22, 2018·Animal : an International Journal of Animal Bioscience·S Fair, P Lonergan
Jun 2, 2017·Reproduction, Fertility, and Development·S A HoldenS Fair
Mar 20, 2018·Journal of Dairy Science·Edel M MurphySean Fair
Oct 24, 2019·BMC Veterinary Research·Kirsten HahnAxel Wehrend
Aug 18, 2021·Animal Reproduction Science·M WiebkeM Schulze

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Statistical Package for the Social Sciences ( SPSS
SPSS
Guavasoft

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