DOI: 10.1101/497016Dec 17, 2018Paper

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-Rot Enterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

BioRxiv : the Preprint Server for Biology
Divine Yutefar ShyntumLucy Moleleki

Abstract

Plant microbial communities' complexity provide a rich model for investigation on biochemical and regulatory strategies involved in interbacterial competition. Within these niches, the soft rot Enterobacteriaceae (SRE) comprise an emerging group of plant-pathogens inflicting soft rot/black-leg diseases and causing economic losses worldwide in a variety of crops. In this report, a range of molecular and computational techniques are utilized to survey the contribution of antimicrobial factors such as bacteriocins, carbapenem antibiotic and type VI secretion system (T6SS) in interbacterial competition among plant-pathogens/endophytes using an aggressive SRE as a case study ( Pectobacterium carotovorum subsp. brasiliense strain PBR1692 — Pcb 1692). A preliminary screening using next-generation sequencing of 16S rRNA comparatively analysing healthy and diseased potato tubers, followed by in vitro competition assays, corroborated the aggressiveness of Pcb 1692 against several relevant taxa sharing this niche ranging from Proteobacteria to Firmicutes . The results showed growth inhibition of several Proteobacteria by Pcb 1692 depends either on carbapenem or pyocin production. Whereas for targeted Firmicutes , only pyocin seems to play...Continue Reading

Related Concepts

Antibiotics
Carbapenems
Case-Control Studies
Enterobacteriaceae
Environment
Gene Deletion
Genes
Genes, Regulator
Genome
Oxygen

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