The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane.

The Journal of Biological Chemistry
M KübrichA Hönlinger

Abstract

The ADP/ATP carrier (AAC) of the mitochondrial inner membrane is synthesized in the cytosol without a cleavable presequence. The preprotein preferentially binds to the mitochondrial surface receptor Tom70 and joins the import pathway of presequence-carrying preproteins at the cis side of the outer membrane. Little is known about the translocation of the AAC across the outer membrane and where its import route separates from that of cleavable preproteins. Here we have characterized a translocation intermediate of AAC during transfer across the outer membrane. The major portion of the preprotein is exposed to the intermembrane space, while a short segment is still accessible to externally added protease. This intermediate can be quantitatively chased to the fully imported form in the inner membrane. Its accumulation depends on Tom7, but not on the intermembrane space domain of Tom22 in contrast to cleavable preproteins. Moreover, opening of the intermembrane space inhibits the import of AAC, but not that of cleavable preproteins into mitoplasts. We conclude that the import route of AAC diverges from the general import pathway of cleavable preproteins already at the trans side of the outer membrane.

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Citations

Nov 5, 1999·Biochimica Et Biophysica Acta·W VoosN Pfanner
May 2, 2001·Nature Reviews. Molecular Cell Biology·N Pfanner, A Geissler
Jan 16, 1999·The EMBO Journal·A AdamM Brunner
Dec 14, 1999·Biological Chemistry·K N Truscott, N Pfanner
Feb 25, 2014·Mitochondrion·Miriam Vázquez-AcevedoDiego González-Halphen
Aug 31, 2002·Cell Communication & Adhesion·Angela M EdmonsonGeorge Argyropoulos
Dec 26, 2009·Mitochondrion·Erika Fernández-VizarraJosé Antonio Enríquez
Jun 13, 2006·Journal of Structural Biology·Claudia LeidholdWolfgang Voos
Mar 20, 2012·Biochimica Et Biophysica Acta·Alejandra Jiménez-SuárezDiego González-Halphen
Feb 14, 2015·Journal of Molecular Biology·Walter Neupert
Apr 18, 2006·Trends in Biochemical Sciences·Carine de Marcos-LousaKostas Tokatlidis
Jun 9, 2006·The Journal of Biological Chemistry·Chris MeisingerNikolaus Pfanner
Apr 28, 2007·The Journal of Biological Chemistry·Pablo M V PeixotoMaría Luisa Campo
Jul 10, 1999·The Journal of Biological Chemistry·M T RyanN Pfanner
Nov 14, 2000·The Journal of Biological Chemistry·A M MeloA Videira
Oct 16, 2002·The Journal of Biological Chemistry·Xuemin XuMei-Zhen Cui
Feb 13, 2003·The Journal of Biological Chemistry·Jeffrey W Priest, Stephen L Hajduk
Feb 24, 2001·Traffic·J Rassow, N Pfanner
Oct 7, 2020·International Journal of Molecular Sciences·Sebastian Kreimendahl, Joachim Rassow

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