The importance of a single amino acid substitution in reduced red blood cell carbonic anhydrase function of early-diverging fish.

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Angelina M DichieraAndrew J Esbaugh

Abstract

In most vertebrates, red blood cell carbonic anhydrase (RBC CA) plays a critical role in carbon dioxide (CO2) transport and excretion across epithelial tissues. Many early-diverging fishes (e.g., hagfish and chondrichthyans) are unique in possessing plasma-accessible membrane-bound CA-IV in the gills, allowing some CO2 excretion to occur without involvement from the RBCs. However, implications of this on RBC CA function are unclear. Through homology cloning techniques, we identified the putative protein sequences for RBC CA from nine early-diverging species. In all cases, these sequences contained a modification of the proton shuttle residue His-64, and activity measurements from three early-diverging fish demonstrated significantly reduced CA activity. Site-directed mutagenesis was used to restore the His-64 proton shuttle, which significantly increased RBC CA activity, clearly illustrating the functional significance of His-64 in fish red blood cell CA activity. Bayesian analyses of 55 vertebrate cytoplasmic CA isozymes suggested that independent evolutionary events led to the modification of His-64 and thus reduced CA activity in hagfish and chondrichthyans. Additionally, in early-diverging fish that possess branchial CA-IV,...Continue Reading

References

Jun 1, 1968·The Journal of Physiology·R E Forster, J B Steen
Jun 1, 1980·The Journal of Physiology·T H Maren, E R Swenson
Jul 1, 1994·Radiology·I M KellyW R Lees
Mar 1, 1993·Respiration Physiology·M Nikinmaa
Apr 4, 2000·Physiological Reviews·C Geers, G Gros
Mar 28, 2001·EXS·S Lindskog, D N Silverman
Jul 4, 2001·Physiological and Biochemical Zoology : PBZ·K M GilmourC M Wood
Feb 5, 2002·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kathleen M GilmourCheryl Szebedinszky
Feb 25, 2003·Molecular Biology and Evolution·Naoko TakezakiJan Klein
Feb 28, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K M Gilmour, S F Perry
Dec 16, 2004·Journal of Inorganic Biochemistry·Thomas Brittain
Feb 17, 2006·Respiratory Physiology & Neurobiology·Michael Berenbrink
May 9, 2006·Respiratory Physiology & Neurobiology·A J Esbaugh, B L Tufts
Sep 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K M GilmourS F Perry
May 12, 2009·Respiratory Physiology & Neurobiology·Andrew J EsbaughS F Perry
May 19, 2009·The Journal of Experimental Biology·K M Gilmour, S F Perry
Jun 15, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K M Gilmour
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
Mar 22, 2012·Database : the Journal of Biological Databases and Curation·Qinghua WangUNKNOWN North East Bioinformatics Collaborative Curation Team
Jan 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M Sun, H Corke
Apr 20, 2014·The Journal of Experimental Biology·D J RandallC J Brauner
May 26, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A J EsbaughM Grosell
May 19, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alexander M CliffordGreg G Goss
May 30, 2019·Proceedings. Biological Sciences·T S HarterC J Brauner
Oct 1, 2019·Physiological and Biochemical Zoology : PBZ·Olivia J L McMillanColin J Brauner

❮ Previous
Next ❯

Citations

Nov 28, 2020·The Journal of Experimental Biology·Angelina M Dichiera, Andrew J Esbaugh

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
restriction
restriction digests

Software Mentioned

SigmaPlot
MEGA7
MrBayes
ClustalX

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.