PMID: 9547274Jun 20, 1998Paper

The importance of base pairing in the penultimate stem of Escherichia coli 16S rRNA for ribosomal subunit association

Nucleic Acids Research
M A Firpo, A E Dahlberg

Abstract

The influence of base pairing in the penultimate stem of Escherichia coli 16S rRNA (defined as nt 1409-1491) on ribosome function has been addressed by the construction of mutations in this region of rRNA. Two sets of mutations were made on either side of a structurally conserved region in the penultimate stem that disrupted base pairing, while a third set of mutations replaced the wild-type sequence with other base pair combinations. The effects of these mutations were analyzed in vivo and in vitro . The mutations that disrupted base pairing caused significant increases in cell doubling times as well as a severe subunit association defect and a modest increase in frame shifting and stop codon read-through. Restoration of base pairing restored wild-type growth rates, decoding and subunit association, indicating that base pairing in this region is essential for proper ribosome function.

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Citations

Dec 13, 2006·Applied and Environmental Microbiology·Federico M LauroDouglas H Bartlett
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·M O'ConnorA E Dahlberg
May 22, 2007·Biochimica Et Biophysica Acta·Anton Vila-SanjurjoMichael O'Connor
Aug 8, 2006·The Journal of Biological Chemistry·Aivar Liiv, Michael O'Connor

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