The importance of cationic amino acid transporter expression in human skin

The Journal of Investigative Dermatology
Oliver SchnorrVictoria Kolb-Bachofen

Abstract

Inducible nitric oxide synthase and arginase activities are acknowledged as important players in human skin epidermal function. For proper enzyme function the substrate availability of L-arginine for both enzymes and thus its transport across the cell membrane via the y+-system (also named cationic amino acid transporters) is critical. Here, we examine the expression of cationic amino acid transporters and their functional role in modulating inducible nitric oxide synthase and arginase activities in human skin and primary keratinocytes, fibroblasts and endothelial cells as well as their impact on keratinocyte proliferation. Skin biopsies were found to express constitutively both cationic amino acid transporter-1 and cationic amino acid transporter-2 mRNA, an expression pattern known to occur in hepatocytes and muscle cells only. To determine the cellular components expressing cationic amino acid transporter, we analyzed the expression patterns in the different human skin cell types in vitro, i.e., in fibroblasts, dermal endothelial cells, and keratinocytes as well as in the HaCaT cell line. An ubiquitous cationic amino acid transporter-1 mRNA expression was found in all cells, whereas constitutive cationic amino acid transporte...Continue Reading

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Citations

Oct 6, 2004·Annual Review of Nutrition·Maria HatzoglouEllen Closs
Dec 17, 2003·The Journal of Investigative Dermatology·Kristin ReckerJohannes Wohlrab
Jul 15, 2016·Mediators of Inflammation·Yung-Ray HsuTzu-Yun Kao
Aug 16, 2014·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Saadet Mercimek-MahmutogluEva Mamak
May 26, 2017·American Journal of Veterinary Research·John P LoftusTomasina A Lucia
Jan 18, 2006·Nitric Oxide : Biology and Chemistry·Guido LerzynskiVictoria Kolb-Bachofen
Jan 4, 2008·Histochemistry and Cell Biology·Kristin JaegerJohannes Wohlrab
Jul 28, 2011·Medicinal Research Reviews·Jonathan McConathyMark M Goodman
Mar 10, 2015·International Journal of Molecular Sciences·Justus WeberWalter Mier
May 7, 2020·Trends in Molecular Medicine·Danay CibrianFrancisco Sánchez-Madrid
Jun 29, 2005·Molecular Pharmaceutics·Laszlo OtvosDavid C Jackson

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