The importance of chromogranin A in the development and function of endocrine pancreas

Regulatory Peptides
G M Portela-GomesSushil K Mahata

Abstract

Chromogranin (Cg) A is expressed in neuroendocrine and neuronal tissues. It is involved in the generation of secretory granules and is cleaved to form biologically active peptides. Targeted ablation of the Chga gene resulted in increased plasma catecholamines, high blood pressure, and decreased size and number of adrenal medullary chromaffin granules. The aim of this study was to determine whether Chga null mice display changes in the morphology and function of the endocrine pancreas. Sections of pancreata from Chga-/-, Chga+/- and Chga+/+ mice, were immunostained with antibodies against synaptophysin, CgA, CgB, secretogranin II and the four major pancreatic islet hormones. Plasma was analysed for glucose, insulin, glucagon, somatostatin and pancreatic polypeptide (PP). CgA epitopes were undetectable in the islets of Chga-/- animals. CgB and secretogranin II epitopes were expressed in the islets of all animal groups albeit with decreased expression in Chga-/- islets. The islet number and size were decreased in the Chga-/- animals compared with Chga+/+. The proportion of insulin cells was decreased but somatostatin and PP cells were increased in Chga-/- mice compared to Chga+/+ mice. The nuclear size was decreased in insulin cel...Continue Reading

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Citations

Feb 4, 2010·PloS One·Stefanie ObermüllerSebastian Barg
Feb 9, 2010·Nature Immunology·Brian D StadinskiKathryn Haskins
Feb 12, 2013·Journal of the American Society for Mass Spectrometry·Gwendoline Thiery-LavenantRichard M Caprioli
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May 6, 2021·Metabolites·Nicholas NorrisMelkam Alamerew Kebede

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