PMID: 2120679Oct 11, 1990Paper

The importance of the 3'-enhancer region in immunoglobulin kappa gene expression

Nucleic Acids Research
K B MeyerM S Neuberger

Abstract

The first enhancers to be identified in the immunoglobulin gene loci are located in the J-C intron. However, deletion of the immunoglobulin kappa intron-enhancer has little effect on the transcription of kappa transgenes. Here we ask whether the second kappa enhancer which we recently identified at the 3'-end of the locus plays a role in kappa gene expression. We show that its omission leads to 20-40 fold lower expression of kappa transgenes and to poor allelic exclusion. Transfection experiments show that activity of the 3'-enhancer, like that of the kappa-intron enhancer, can be induced in a pre-B cell line by incubation with bacterial lipopolysaccharide. Whereas induction of the kappa-intron enhancer is due to induction of NF-kappa B activity, deletion mapping of the 3'-enhancer localises its activity to a 50 nucleotide region that lacks an NF-kappa B site; indeed the 3'-enhancer allows kappa expression in a cell line which lacks NF-kappa B. Thus, both the 3'- and intron-enhancers can be induced at the same stage of differentiation but by distinct pathways. Furthermore, unlike the intron-enhancer, the 3'-enhancer plays a critical role in the transcription of rearranged immunoglobulin kappa genes.

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Citations

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K Park, M L Atchison
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A González-Fernández, C Milstein
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Bachl, M Wabl
Oct 3, 2002·The EMBO Journal·Maya GoldmitYehudit Bergman
Mar 2, 2005·Seminars in Immunology·Anne E Corcoran
Jun 7, 2014·Frontiers in Immunology·Rena Levin-KleinYehudit Bergman
Nov 1, 1995·European Journal of Immunology·R MocikatH G Klobeck
Jun 1, 1991·European Journal of Immunology·P DariavachM S Neuberger
Sep 14, 2001·Scandinavian Journal of Immunology·J WrammertT Leanderson
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel C McDevitBarbara S Nikolajczyk
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·X LiuB Van Ness
Mar 4, 1998·European Journal of Immunology·Y YedidiaS Jung
Jun 1, 1996·Molecular and Cellular Biology·M C RoqueV C Blasquez

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