The importance of the membrane for biophysical measurements.

Nature Chemical Biology
Dror S Chorev, Carol V Robinson

Abstract

Within cell membranes numerous protein assemblies reside. Among their many functions, these assemblies regulate the movement of molecules between membranes, facilitate signaling into and out of cells, allow movement of cells by cell-matrix attachment, and regulate the electric potential of the membrane. With such critical roles, membrane protein complexes are of considerable interest for human health, yet they pose an enduring challenge for structural biologists because it is difficult to study these protein structures at atomic resolution in in situ environments. To advance structural and functional insights for these protein assemblies, membrane mimetics are typically employed to recapitulate some of the physical and chemical properties of the lipid bilayer membrane. However, extraction from native membranes can sometimes change the structure and lipid-binding properties of these complexes, leading to conflicting results and fueling a drive to study complexes directly from native membranes. Here we consider the co-development of membrane mimetics with technological breakthroughs in both cryo-electron microscopy (cryo-EM) and native mass spectrometry (nMS). Together, these developments are leading to a plethora of high-resolut...Continue Reading

References

Aug 5, 2000·Science·K PalczewskiM Miyano
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Manuela ZoonensJean-Luc Popot
Jun 17, 2008·Science·Nelson P BarreraCarol V Robinson
Jun 24, 2010·Current Opinion in Structural Biology·Thomas RaschleGerhard Wagner
Mar 23, 2011·Nature·Feiran LuNieng Yan
Jul 21, 2011·Nature·Søren G F RasmussenBrian K Kobilka
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lindsay A BakerJohn L Rubinstein
Aug 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen M DaviesWerner Kühlbrandt
Oct 16, 2012·Nature Methods·Rebecca J RoseAlbert J R Heck
May 27, 2014·The Journal of Membrane Biology·Shantha ElterGerhard Wagner
May 29, 2014·Nature Communications·Tzviya Zeev-Ben-MordehaiKay Grünewald
Aug 12, 2014·The FEBS Journal·Nikolaj D DrachmannMaike Bublitz
Dec 17, 2014·Analytical Chemistry·Antonio N CalabreseAlison E Ashcroft
Jan 24, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Martin Caffrey
Jan 9, 2016·Science·Jeremy BakelarNicholas Noinaj
Feb 24, 2016·Nature Structural & Molecular Biology·Long HanYihua Huang
Feb 24, 2016·Nature Methods·Joseph GaultCarol V Robinson
Mar 8, 2016·Nature Methods·Jens FrauenfeldPär Nordlund
Jul 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Idlir LikoCarol V Robinson
Sep 23, 2016·Nature·Hervé CeliaSusan K Buchanan
Oct 1, 2016·Nature Communications·Matthew G IadanzaNeil A Ranson
Oct 11, 2016·Current Opinion in Structural Biology·Idlir LikoCarol V Robinson
Nov 11, 2016·ELife·Joana S SousaWerner Kühlbrandt
Jan 13, 2017·Nature·Kallol GuptaCarol V Robinson
Jul 5, 2017·Current Opinion in Structural Biology·Radostin Danev, Wolfgang Baumeister
Oct 11, 2017·Journal of the American Chemical Society·Chih-Ta Henry ChienDaniel Nietlispach
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·M R SandersP J Booth

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Citations

Dec 31, 2020·International Journal of Molecular Sciences·Johannes Thoma, Björn M Burmann
May 1, 2021·Current Opinion in Structural Biology·Mark T Agasid, Carol V Robinson
Jul 17, 2021·Frontiers in Molecular Biosciences·James S DaviesRenwick C J Dobson

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Methods Mentioned

BETA
X-ray
NMR
SMA

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