PMID: 9435214Jan 22, 1998Paper

The importance of tRNA backbone-mediated interactions with synthetase for aminoacylation

Proceedings of the National Academy of Sciences of the United States of America
W H McClainK Gabriel

Abstract

We have identified six new aminoacylation determinants of Escherichia coli tRNAGln in a genetic and biochemical analysis of suppressor tRNA. The new determinants occupy the interior of the acceptor stem, the inside corner of the L shape, and the anticodon loop of the molecule. They supplement the primary determinants located in the anticodon and acceptor end of tRNAGln described previously. Remarkably, the three-dimensional structure of the complex between tRNAGln and glutaminyl-tRNA synthetase shows that the enzyme interacts with the phosphate-sugar backbone but not the base of every new determinant. Moreover, a small protein motif interacts with five of these determinants, and it binds proximal to the sixth. The motif also interacts with the middle base of the anticodon and with the backbones of six other nucleotides. Our results emphasize that synthetase recognition of tRNA is more elaborate than amino acid side chains of the enzyme interacting with nucleotide bases of the tRNA. Recognition also includes synthetase interaction with tRNA backbone functionalities whose distinctive locations in three-dimensional space are exquisitely determined by the tRNA sequence.

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Citations

Dec 4, 2002·Journal of Molecular Biology·Hyunsic ChoiWilliam H McClain
Aug 13, 1998·RNA·A D WolfsonO C Uhlenbeck
Mar 24, 2009·Nature Structural & Molecular Biology·Sarah LedouxOlke C Uhlenbeck
Aug 24, 2005·Nature Structural & Molecular Biology·Mikołaj OlejniczakOlke C Uhlenbeck
Nov 4, 1998·Nucleic Acids Research·R GiegéC Florentz
Feb 9, 2010·Nucleic Acids Research·Matthieu G GagnonSergey V Steinberg
Jul 5, 2006·Journal of Bioinformatics and Computational Biology·Swetlana NikolajewaThomas Wilhelm
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·William H McClain
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·K Y ChangW H McClain
Sep 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nathan T Uter, John J Perona
Sep 25, 1999·Journal of Molecular Biology·P Auffinger, E Westhof
Dec 1, 2009·FEBS Letters·David H Ardell
Aug 31, 2000·Annual Review of Biochemistry·M Ibba, D Soll
Dec 23, 2009·Journal of Molecular Biology·Renaud Geslain, Tao Pan
Jul 11, 2008·Molecular Cell·Sarah Ledoux, Olke C Uhlenbeck
Sep 16, 2005·The Journal of Biological Chemistry·Hervé RoyMichael Ibba
Jul 15, 2015·Nature Communications·Jonathan W CruzNancy A Woychik
May 16, 2019·Journal of Biomolecular Structure & Dynamics·Mutharasappan NachiappanJeyaraman Jeyakanthan
May 5, 2020·Frontiers in Genetics·Dukas Jurėnas, Laurence Van Melderen
Jul 3, 1999·Journal of Molecular Biology·W H McClainJ Schneider
May 10, 2000·Journal of Molecular Biology·G Tocchini-ValentiniJ Abelson

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