The in vitro activity of selected defensins against an isolate of Pseudomonas in the presence of human tears.

The British Journal of Ophthalmology
Alison M McDermottC J Murphy

Abstract

Pseudomonas aeruginosa is a major cause of severe bacterial keratitis and remains a difficult clinical entity to treat successfully with the current arsenal of antimicrobial agents. Defensins are small cationic peptides with broad in vitro antimicrobial activity and are potential ocular therapeutic agents. The authors characterised the in vitro activity of defensins NP-1 and NP-3a against P aeruginosa in the presence of human tears. A clinical Pseudomonas isolate was grown to mid-log phase, and 1 x 10(6 )colony forming units were exposed to the peptides (200 microg/ml) for up to 2 hours in the presence of varying concentrations (10-70%) of human tears. For both peptides in the presence of 10% tears, >3 log units of killing was achieved within 30 minutes. In 70% tears, NP-1 produced >1 log unit of killing at 2 hours, indicating that, although reduced, its activity remained significant. In 20% tears, NP-3a demonstrated 2 log units of killing at 2 hours; however, the antimicrobial activity of this defensin was completely inhibited in the presence of 70% tears. These in vitro data suggest that while the microbicidal activity of some defensins may be diminished at the ocular surface in vivo, significant activity is still possible wi...Continue Reading

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Citations

May 18, 2011·Cellular and Molecular Life Sciences : CMLS·Satya S Kolar, Alison M McDermott
Aug 29, 2007·Antimicrobial Agents and Chemotherapy·Ling C HuangAlison M McDermott
May 5, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David B AlexanderHiroyuki Tsuda
Sep 25, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Curtis R Brandt
Aug 5, 2014·Cellular and Molecular Life Sciences : CMLS·Satya Sree N KolarMaria Luisa Mangoni
Sep 14, 2007·Current Eye Research·Ling C HuangAlison M McDermott
Apr 16, 2013·The Ocular Surface·Srihari NarayananAlison M McDermott
May 12, 2015·Peptides·Maria Luisa MangoniAlison M McDermott
Feb 5, 2015·Veterinary Immunology and Immunopathology·Tina S DalgaardHelle R Juul-Madsen
May 15, 2007·Current Opinion in Infectious Diseases·Philip Aloysius Thomas, Pitchairaj Geraldine
Sep 23, 2010·Current Eye Research·Xiaoyong YuanKirk R Wilhelmus
Sep 15, 2020·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Shyam Sunder Tummanapalli, Mark Dp Willcox

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