PMID: 9168236May 20, 1997Paper

The in vitro anti-HIV efficacy of negatively charged human serum albumin is antagonized by heparin

AIDS Research and Human Retroviruses
P J SwartD K Meijer

Abstract

Succinylated human serum albumin (Suc-HSA) was synthesized by treating human serum albumin with succinic anhydride. Among similar proteins and neo(glyco)proteins tested, Suc-HSA exhibits a pronounced net negative charge, a feature that largely contributes to its efficacy against replication of human immunodeficiency virus type 1 (HIV-1). To assess further the antiviral effect of Suc-HSA, the effect on HIV-1 replication was studied in the presence of whole human plasma. Pretreatment of MT2 cells with Suc-HSA was more efficacious than direct Suc-HSA treatment of HIV prior to addition to the cells. No changes in the antiviral effect of Suc-HSA were observed in tissue culture medium, 30% plasma, or whole plasma when CPDA-1 (citrate-phosphate-dextrose-adenine 1) was used as the anticoagulant. However, a dramatic decrease (greater than 99%) in the antiviral activity was observed when these experiments were performed in plasma prepared from blood using heparin as anticoagulant. The antagonistic effect by heparin was observed both in the case that heparin was added prior to or after addition of Suc-HSA to the test system. In the present study we demonstrate that heparin largely reduces Suc-HSA activity on HIV replication in the same co...Continue Reading

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Citations

May 13, 1999·Antiviral Research·M E BursteinA G Bukrinskaya
Mar 16, 2012·Journal of Food Science·Athanasia O MatemuSoichiro Nakamura
Nov 22, 2002·Journal of Enzyme Inhibition and Medicinal Chemistry·Valerie E DoylIan H Gilbert

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