The in vitro comparative study of the effect of BPA, BPS, BPF and BPAF on human erythrocyte membrane; perturbations in membrane fluidity, alterations in conformational state and damage to proteins, changes in ATP level and Na(+)/K(+) ATPase and AChE activities

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Aneta MaćczakJaromir Michałowicz

Abstract

Bisphenols are massively used in the industry, and thus the exposure of biota including humans to these substances has been noted. In this study we have assessed the effect of BPA and its selected analogs, i.e. BPS, BPF and BPAF on membrane of human red blood cells, which is the first barrier that must be overcome by xenobiotics penetrating the cell, and is commonly utilized as a model in the investigation of the effect of different xenobiotics on various cell types. Red blood cells were incubated with BPA and its analogs in the concentrations ranging from 0.1 to 250 μg/ml for 4 h and 24 h. We have noted that the compounds studied altered membrane fluidity at its hydrophobic region, increased internal viscosity and osmotic fragility of the erythrocytes and altered conformational state of membrane proteins. Moreover, bisphenols examined increased thiol groups level, caused oxidative damage to membrane proteins, decreased ATP level, depleted the activity of Na+/K + ATPase and changed the activity of AChE in human red blood cells. It has been shown that the strongest changes were noted in cells treated with BPAF, while BPS caused the weakest (or none) alterations in the parameters studied.

Citations

Jun 4, 2020·International Journal of Molecular Sciences·Monika JarosiewiczBożena Bukowska
Dec 10, 2019·Colloids and Surfaces. B, Biointerfaces·Beata WyżgaKatarzyna Hąc-Wydro
Jul 14, 2020·The Science of the Total Environment·Yongna YuanChunyan Zhao
Nov 20, 2020·Environmental Research·Eve PellerinMartin Pelletier
Apr 9, 2018·Chemosphere·Monika Zbucka-KretowskaMichal Ciborowski
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Piotr DuchnowiczBożena Bukowska
Jul 12, 2018·Environmental Science & Technology·Hualing FuBin Zhao

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