The in vitro development of bovine oocytes after maturation in glucose and beta-hydroxybutyrate concentrations associated with negative energy balance in dairy cows

Reproduction in Domestic Animals = Zuchthygiene
J L M R LeroyA de Kruif

Abstract

Negative energy balance (NEB) in high yielding dairy cows early postpartum may affect oocyte quality. Therefore, we tested the effect of two different beta-hydroxybutyrate (BHB) and glucose concentrations, which are associated with subclinical or clinical ketosis, during in vitro maturation (IVM) on the developmental competence of bovine oocytes. In Expt 1, subclinical ketosis conditions were imitated. Oocytes were matured in four different serum-free media with two glucose concentrations (g1=2.75 mm or G1=5.5 mm glucose) and with or without BHB (BHB1=1.8 mm BHB). Following maturation groups were used: g1, G1, g1:BHB1 and G1:BHB1. In Expt 2, clinical ketosis conditions were mimicked by using the concentrations: g2=1.375 mm or G2=3.1 mm glucose and BHB2=4.0 mm BHB. The combinations used were: g2, G2, g2:BHB2 and G2:BHB2. After IVM and in vitro fertilization (IVF), presumptive zygotes were routinely cultured for 7 days in synthetic oviduct fluid [SOF; 5% fetal calf serum (FCS)]. At 48 h and 8 days pi, cleavage rate and number of blastocysts were recorded respectively. The results demonstrated that the maturation conditions mimicking subclinical (g1:BHB1) and clinical ketosis (g2 : BHB2) resulted in an impaired developmental compe...Continue Reading

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Citations

Aug 1, 2008·Animal : an International Journal of Animal Bioscience·J L M R LeroyP E J Bols
Sep 15, 2012·The Journal of Reproduction and Development·Safiriyu Idowu Ola, Qing-Yuan Sun
Jan 8, 2013·The Journal of Reproduction and Development·Borjigin SarentonglagaYoshikazu Nagao
Dec 27, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·M A FenwickD C Wathes
May 23, 2014·Animal : an International Journal of Animal Bioscience·J K Drackley, F C Cardoso
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·J L M R LeroyP E J Bols
May 11, 2016·Theriogenology·J E P SantosE S Ribeiro
Feb 6, 2004·Journal of Dairy Science·C Schlumbohm, J Harmeyer
Mar 21, 2016·Journal of Dairy Science·Andrew J RutherfordRobert F Smith
May 11, 2010·Reproduction, Fertility, and Development·Cheryl J SchelbachJeremy G Thompson
Jan 1, 2019·Reproduction, Fertility, and Development·M MikkolaJ Taponen
Aug 5, 2020·Journal of Dairy Science·E E A BurgersA T M van Knegsel
Aug 18, 2020·Journal of Animal Science and Technology·Sung-Sik KangSang-Rae Cho
Nov 22, 2020·Animals : an Open Access Journal From MDPI·Robert MikułaWłodzimierz Nowak

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