The in vitro effect of Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide on murine peritoneal macrophages

Canadian Journal of Microbiology
K A Barker, S C Holt

Abstract

Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide (LPS) was examined for its in vitro effect on murine peritoneal macrophage morphology, viability, and lysosomal enzyme activity. Fifty micrograms of Y4 LPS per 10(6) macrophages resulted in macrophage activation, eliciting the release of acid phosphates (AcP), as well as the accumulation of intracellular AcP, without a loss in viability. There was also an increase in the number of organelles and cell-surface ruffles. One hundred or 250 microgram of Y4 LPS caused flattening, rounding, and blebbing of the cell surface, as well as the release of large quantities of AcP and lactate dehydrogenase (LDH), within 5 min of exposure to the LPS. Alteration of cell ultrastructure occurred within 30 min, and extensive autophagocytosis by 24 h, indicative of cell injury. Short-term (less than 24 h) experiments appear necessary to distinguish between the effects of cell activation and cell death caused by A. actinomycetemcomitans Y4 LPS.

Citations

Dec 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A VesselinovaH Najdenski
Sep 14, 2001·Oral Microbiology and Immunology·M Fukunaga, K Tsuruda
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S C Holt, T E Bramanti
Dec 1, 1987·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·B JohneB Mørland

Related Concepts

Acid Phosphatase
Actinobacillus
Metazoa
Cell Survival
Lactate Dehydrogenase
Lipopolysaccharides
Lysosomes
Macrophage
Mice, Inbred Strains
Electron Microscopy

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