The in vitro effect of lipopolysaccharide on proliferation, inflammatory factors and antioxidant enzyme activity in bovine mammary epithelial cells

Animal Nutrition
Huiyu ShiS M Yan

Abstract

Lipopolysaccharide (LPS) was selected as a stimulus to investigate its effect on cell viability and oxidative stress in bovine mammary epithelial cells (BMEC) by detecting the cell relative growth rate (RGR), antioxidant indicators and inflammatory factors. This information was used to provide the theoretical basis for the establishment of a LPS-induced oxidative damage model. The experiment was divided into two parts. The first part used a two-factor experimental design to determine the appropriate incubation time of LPS by detecting the RGR. The third-passage BMEC were divided into 24 groups with six replicates in each group. The first factor was LPS concentration, which was 0 (control), 0.1, 1.0 and 10.0 μg/mL; the second factor was LPS incubation time (2, 4, 6, 8, 12 and 24 h). The optimum LPS incubation time was 6 h according to the results of the first part of the experiment. The second part of the experiment was conducted using a single-factor experimental design, and the third-passage cells were divided into four groups with six replicates in each group. The cells were incubated with culture medium containing different concentrations of LPS (0 [control], 0.1, 1.0 and 10.0 μg/mL) for 6 h to select the appropriate concent...Continue Reading

Citations

Mar 27, 2020·Journal of Cardiovascular and Thoracic Research·Mina MamipourReza Rahbarghazi
Nov 19, 2019·Ocular Immunology and Inflammation·Deniz MarangozAfsun Sahin
Sep 1, 2018·Journal of Materials Science. Materials in Medicine·Lucie VálkováAntonín Dlouhý
Oct 9, 2021·Scientific Reports·Bartosz SikoraJoanna Gola

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