The in vitro effects of Trolox on methylmercury-induced neurotoxicity

Toxicology
Parvinder KaurTore Syversen

Abstract

Methylmercury (MeHg), an environmental toxicant primarily found in fish and seafood poses a dilemma to both consumers and regulatory authorities given the nutritional benefits of fish consumption vs. possible adverse neurological damage caused by MeHg. The present study addresses whether supplementation with 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), alters the neuro-oxidative effects of MeHg in C6-glioma and B35-neuronal cell lines. As indicators of cytotoxicity, reduced glutathione (GSH), reactive oxygen species (ROS) and mitochondrial activity (MTT) were measured. The cellular mercury (Hg) content was measured with high resolution-inductively coupled plasma mass spectrometry (HR-ICPMS). The amount of MeHg-induced ROS was significantly reduced (p<0.05) after treatment with 50muM Trolox in C6 glial cell line. However, treatment with Trolox did not induce any significant increase in GSH levels or MTT activity in either of the cell lines. In addition, treatment with Trolox did not induce any significant changes in intracellular MeHg levels. The MeHg and Trolox treated C6 glial cell line differed significantly (p<0.05) from the B35 cell line for MTT, ROS and GSH activity. These findings provide experimental...Continue Reading

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Citations

Jun 5, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Tore Syversen, Parvinder Kaur
May 24, 2011·Toxicology and Applied Pharmacology·Marcelo FarinaJoão B T Rocha
Sep 24, 2011·Journal of Toxicology·Parvinder KaurTore Syversen
Oct 7, 2011·Journal of Biomedical Materials Research. Part a·Paritosh P WattamwarThomas D Dziubla
Oct 21, 2014·PloS One·Allan Costa-MalaquiasMaría Elena Crespo-Lopez

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