The in vitro impact of toothpaste extracts on cell viability

European Journal of Oral Sciences
Barbara CviklReinhard Gruber

Abstract

Toothpastes contain three main components: detergents, abrasives, and fluoride. Detergents, particularly sodium lauryl sulfate, have been proposed as components that enable toothpastes to produce cytotoxic effects in vitro. However, not all toothpastes contain sodium lauryl sulfate, and almost no studies have found an association between detergents and the in vitro cytotoxicity of toothpastes. The present study examined the in vitro cytotoxicity of nine commercially available toothpastes containing four different detergents. Toothpastes were diluted in serum-free medium, centrifuged, and filter sterilized. The half-lethal concentration of the toothpaste-conditioned medium (TCM) was calculated based on the formation of formazan by gingival fibroblasts, oral squamous cell carcinoma HSC-2 cells, and L929 cells. Cell proliferation was analyzed, and live-dead staining was performed, after exposure of cells to conditioned medium prepared with 1% toothpaste (1% TCM). It was found that toothpastes containing sodium lauryl sulfate and amine fluoride strongly inhibited cell viability with the half-lethal concentration being obtained with conditioned medium prepared with approximately 1% toothpaste (1% TCM). Toothpastes containing cocamid...Continue Reading

References

Feb 1, 1996·European Journal of Oral Sciences·B B Herlofson, P Barkvoll
Jun 1, 1996·Acta Odontologica Scandinavica·B B Herlofson, P Barkvoll
Nov 1, 1996·Journal of Clinical Periodontology·B B HerlofsonH Aars
Jul 21, 1998·Acta Odontologica Scandinavica·E SöderlingJ Tenovuo
Jun 1, 2001·Laryngo- rhino- otologie·N H KleinsasserU A Harréus
Apr 5, 2003·Skin Pharmacology and Applied Skin Physiology·L BenassiS Seidenari
Sep 5, 2008·International Journal of Dental Hygiene·C MooreJ Moran
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanae MatsudaMasahiro Iwaki
Aug 17, 2012·International Journal of Toxicology·Christina L BurnettF Alan Andersen
Sep 29, 2012·Journal of Dental Research·W L SiqueiraE E McDonald
Jan 29, 2013·Cutaneous and Ocular Toxicology·Esin GünerMurat Baykır
Jul 3, 2013·Monographs in Oral Science·Frank Lippert
Feb 11, 2014·Journal of the American Academy of Dermatology·Cristin N ShaughnessyDonald V Belsito

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Citations

Aug 1, 2015·Clinical Oral Investigations·Mariana BrunoFernanda Calabró Calheiros
Apr 8, 2016·Clinical Oral Investigations·Barbara CviklReinhard Gruber
Sep 28, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Agnieszka Machorowska-PieniążekStefan Baron
Dec 8, 2017·Brazilian Dental Journal·Samira Esteves Afonso CamargoCarlos Henrique Ribeiro Camargo
Feb 21, 2019·Acta Odontologica Scandinavica·Antonija TadinDavor Zeljezic
Aug 23, 2020·Toxins·Amanda Pissinatti CanelliMaurício Ventura Mazzi
Dec 26, 2018·BioMed Research International·Anna Sonja MüllerHermann Agis
Feb 20, 2021·Brazilian Oral Research·Sigmar de Mello RodeSamira Esteves Afonso Camargo

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