The in vitro quality of red blood cells frozen with 40 percent (wt/vol) glycerol at -80 degrees C for 14 years, deglycerolized with the Haemonetics ACP 215, and stored at 4 degrees C in additive solution-1 or additive solution-3 for up to 3 weeks

Transfusion
C R VALERIG Ragno

Abstract

Red blood cells (RBCs) frozen with 40 percent (wt/vol) glycerol, stored at -80 degrees C (mean temperature; range, -65 to -90 degrees C) for 14 years, deglycerolized in the Haemonetics automated cell processor (ACP) 215 with the 325-mL disposable bowl, and stored at 4 degrees C in additive solution (AS)-1 or AS-3 for 21 days were evaluated. A total of 106 units of citrate phosphate dextrose adenine-1 RBCs were frozen with 40 percent (wt/vol) glycerol in the original 800-mL polyvinylchloride plastic bag and stored in corrugated cardboard boxes at -80 degrees C for 14 years. The thawed units were deglycerolized with the ACP 215 with a 325-mL disposable bowl and stored in AS-1 or AS-3 at 4 degrees C for 21 days. The freeze-thaw recovery value was 94 +/- 4 percent (mean +/- SD), the freeze-thaw-wash recovery value was 80 +/- 7 percent, and there was no breakage. Thirty-eight units were processed as 19 pairs. Two units of ABO-matched units were thawed, pooled, divided equally into two units, and deglycerolized. One unit was stored in AS-1 and the other in AS-3 at 4 degrees C for 21 days. Units stored in AS-1 exhibited significantly greater hemolysis than those stored in AS-3. Acceptable results were achieved when RBCs frozen at -80 ...Continue Reading

References

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Citations

Dec 18, 2007·Journal of Trauma Nursing : the Official Journal of the Society of Trauma Nurses·Joseph R SpaniolJanet D Pierce
Sep 28, 2007·Current Opinion in Hematology·Viviana V Johnson, Sean A Swiatkowski
Aug 24, 2011·The Journal of Trauma·Francisco J Rentas
Jan 1, 1994·Vox Sanguinis·A W Rowe
Aug 28, 2014·The Journal of Trauma and Acute Care Surgery·Nicole T Gordon, Martin A Schreiber
Feb 23, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·C Robert Valeri, Gina Ragno
Aug 3, 2010·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Yang ZhaoKejun Huang
Mar 23, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·C Robert Valeri, Gina Ragno
Oct 13, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Gina Ragno, C Robert Valeri
Jul 29, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·C Robert Valeri, Gina Ragno
Jul 4, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·C C M LelkensF Noorman

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