The in vitro serum protein-binding characteristics of bis-(2-ethylhexyl) phthalate and its principal metabolite, mono-(2-ethylhexyl) phthalate

Environmental Health Perspectives
W C GriffithsJ Gentile

Abstract

The metabolism and toxicity of the ubiquitous plasticizer, bis-(2-ethylhexyl) phthalate (DEHP), and its principal metabolite, mono-(2-ethylhexyl) phthalate (MEHP), have been extensively investigated. In an attempt to understand their disposition in man, we studied the in vitro serum protein-binding characteristics of these compounds, using ultracentrifugation and agarose gel electrophoresis. The association of DEHP and lipoproteins was shown to be highly dependent upon, and proportional to, the lipid concentration of the serum. It appears that more than half of the serum DEHP is bound to proteins with density greater than 1.21 g/mL when the concentration of cholesterol is below 300 mg/dL or the cholesterol and triglyceride total concentration is less than 600 mg/dL. As the cholesterol and triglyceride concentrations increase, the percent DEHP bound to VLDL, IDL, and LDL increases. MEHP is bound principally to nonlipoprotein constituents in the serum, and this binding distribution is unaffected by lipid concentration. The percent binding of DEHP and MEHP to individual proteins was also found to be unaffected by their concentrations in serum. These data indicate that the protein-binding characteristics of these compounds, in vitr...Continue Reading

References

Nov 1, 1978·Transfusion·P W Albro, J T Corbett
Dec 1, 1975·JAMA : the Journal of the American Medical Association·K OnoT Wakimoto
Apr 8, 1986·Biochemistry·S Lund-Katz, M C Phillips
Mar 1, 1986·Environmental Health Perspectives·G RockM Tocchi
Jan 3, 1970·Lancet·Y L Marcel, S P Noel
Jul 18, 1970·Lancet·R J Jaeger, R J Rubin
Jun 21, 1973·Biochimica Et Biophysica Acta·P W Albro, R O Thomas
Jan 1, 1984·Critical Reviews in Toxicology·J A Thomas, M J Thomas
Oct 1, 1982·Journal of Toxicology and Environmental Health·W M KluweJ E Huff
Sep 1, 1985·Environmental Monitoring and Assessment·L E Eberhardt, L L Cadwell

❮ Previous
Next ❯

Citations

Jun 1, 1996·Critical Reviews in Toxicology·W W HuberR Schulte-Hermann
Oct 2, 2014·Archives of Toxicology·Jin-Feng ZhaoTzong-Shyuan Lee
Mar 17, 2012·Ecotoxicology and Environmental Safety·Lena OlsénP Monica Lind
Oct 30, 2014·Journal of Applied Toxicology : JAT·Kristina SchaedlichBernd Fischer
Mar 9, 2012·Environmental Health Perspectives·Morten Søndergaard JensenBo A G Jönsson
Feb 24, 2018·Scientific Reports·Kristina SchaedlichJana Ernst
May 15, 2018·Environmental Science & Technology·Xiaoying LiLei Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.