The in vitro synthesis of estrogen-dependent proteins by the baboon (Papio anubis) oviduct

Endocrinology
H G Verhage, A T Fazleabas

Abstract

This study was undertaken to determine the effect of estradiol and progesterone on the synthesis of oviduct-specific proteins and to determine if there were changes in the synthesis of these proteins in different regions of the oviduct. Ovariectomized females were either untreated, treated with estradiol for 7 or 14 days, or primed with estradiol for 14 days and then treated with estradiol plus progesterone or progesterone alone for either 7 or 14 days. Oviducts were incubated in the presence of labeled methionine or glucosamine for 24 h at 37 C, and the culture medium was then analyzed by one- and two-dimensional (2-D) polyacrylamide gel electrophoresis, followed by fluorography. A family of macromolecules [mol wt (Mr), 100,000-130,000] was only present in the animals treated with estradiol for 7 or 14 days. 2-D analysis demonstrated that two proteins, one basic and one acidic, were the major estradiol-responsive proteins in the 100,000-130,000 Mr region. In addition, an acidic protein in this region increased in intensity with estradiol treatment. All three proteins incorporated methionine and glucosamine. Since a steroid-responsive gradient is known to exist in the oviduct, oviducts were divided into fimbria, ampulla, and is...Continue Reading

Citations

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