PMID: 9187032Apr 1, 1997Paper

The in vitro uptake and incorporation of hemoglobin by adult Haemonchus-contortus

Veterinary Parasitology
R H Fetterer, M L Rhoads

Abstract

The incorporation of radioactivity from [3H]leucine-labeled hemoglobin (Hb) into adult Haemonchus contortus proteins was investigated. Further, the role of previously described cysteine proteases present in intestinal tissue and excretory/secretory products of H. contortus was assessed in the breakdown of Hb. A cell lysate preparation (predominantly Hb) was obtained from reticulocytes metabolically labeled, in vitro, with [3H]leucine. Following 24-h incubation in the presence of [3H]Hb, adult H. contortus incorporated radioactivity. The presence of the protein synthesis inhibitor puromycin (200 micrograms ml-1) reduced incorporation by 72%, indicating that this process was dependent on protein synthesis. The specific cysteine protease inhibitor Z-phe-ala-FMK (PAF) at 0.1 mM had no effect on incorporation of radioactivity; however, the breakdown of Hbg in the culture medium was reduced by 50%. In contrast, PAF at 1.0 mM caused a 78% reduction in incorporated radioactivity. Parasite viability was also decreased by 1.0 mM PAF, and thus the reduction of incorporation of radioactivity may not be due to specific enzyme inhibition. The serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) at 1.0 mM caused a 40% re...Continue Reading

References

Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M R KasschauM P Byam-Smith
Dec 1, 1990·Molecular and Biochemical Parasitology·D PrattR J Boisvenue
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R H Fetterer
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Citations

Nov 18, 1997·International Journal for Parasitology·R H Fetterer, M L Rhoads
Nov 1, 2001·Trends in Parasitology·S HashmiS Lustigman
Dec 16, 1998·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·D K KocherM L Sood
Dec 19, 2012·Experimental Parasitology·C Martínez-Ortíz-de-MontellanoH Hoste
Mar 4, 2000·The Journal of Parasitology·R H Fetterer, M L Rhoads

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