The in vitro use of the hair follicle closure technique to study the follicular and percutaneous permeation of topically applied drugs

Alternatives to Laboratory Animals : ATLA
Jessica StahlManfred Kietzmann

Abstract

Recent studies on follicular permeation emphasise the importance of hair follicles as diffusion pathways, but only a limited amount of data are available about the follicular permeation of topically applied drugs. This study examines the use of a hair follicle closure technique in vitro, to determine the participation of hair follicles in transdermal drug penetration. Various substances, with different lipophilicities, were tested: caffeine, diclofenac, flufenamic acid, ibuprofen, paracetamol, salicylic acid and testosterone. Diffusion experiments were conducted with porcine skin, the most common replacement material for human skin, in Franz-type diffusion cells over 28 hours. Different experimental settings allowed the differentiation between interfollicular and follicular permeation after topical application of the test compounds. A comparison of the apparent permeability coefficients of the drugs demonstrates that the percutaneous permeations of caffeine and flufenamic acid were significantly higher along the hair follicles. In the cases of paracetamol and testosterone, the follicular pathway appears to be of importance, while no difference was found between interfollicular and follicular permeation for diclofenac, ibuprofen...Continue Reading

References

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Jul 6, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ulrike Blume-PeytaviNatalie Garcia Bartels
Nov 20, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexa PatzeltJuergen Lademann

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Citations

Jun 9, 2017·Experimental Biology and Medicine·H E AbaciAngela Christiano
Aug 28, 2021·Medical Sciences : Open Access Journal·Eberhard GrambowDaniel Strüder

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